Probiotic Potential of Lactobacilli with Antagonistic Activity Against Pathogenic Strains: An in Vitro Validation for the Production of Inhibitory Substances

Overview

Journal Biomed J

Publisher Elsevier

Specialty General Medicine

Date 2017 Nov 29

PMID 29179882

Citations 62

Authors

Chidre Prabhurajeshwar

Revanasiddappa Kelmani Chandrakanth

Affiliations

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Abstract

Background: Probiotics, live cells with different beneficiary characteristics, have been extensively studied and explored commercially in many different products in the world. Their benefits to human and animal health have proven in hundreds of scientific studies. Based on rich bibliographic material, Curd is the potential source of probiotic Lactobacilli.

Method: The aim of the present study was to observe Lactobacilli with probiotic potential activities from different curd samples for isolation, identification and characterization of Lactobacillus species.

Results: Among the samples, thirty lactic acid bacterial strains were isolated, sixteen (16/30) best Lactobacillus isolates were selected by preliminary screening as potential probiotic for acid and bile tolerance, further confirmed using 16s rRNA identification. All the selected Lactobacillus isolates were then characterized in vitro for their probiotic characteristics and antimicrobial activities against pathogens and aggregation studies. The results indicated that selected potential probiotic isolates (T2, T4 and T16) were screened and confirmed as Lactobacillus. The isolates produced positive tolerance to excited pH, NaCl and bile salts, also revealed noticeable antimicrobial activities against pathogens. All the Lactobacillus isolates were susceptible to clinical antibiotics used. Besides, T2 isolate was constituted to retain stronger auto and co-aggregation and cell surface hydrophobicity capacity.

Conclusion: Based on the drawn results, T2, T4 and T16 Lactobacillus isolates were recognised as ideal, potential in vitro antimicrobial probiotic isolates against pathogens and studies are needed further in-vivo assessment and human health benefits in their real-life situations.

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References

Bravo J, Forsythe P, Chew M, Escaravage E, Savignac H, Dinan T . Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve. Proc Natl Acad Sci U S A. 2011; 108(38):16050-5. PMC: 3179073. DOI: 10.1073/pnas.1102999108. View

BAUER A, KIRBY W, SHERRIS J, Turck M . Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45(4):493-6. View

Lahteinen T, Malinen E, Koort J, Mertaniemi-Hannus U, Hankimo T, Karikoski N . Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe. 2009; 16(3):293-300. DOI: 10.1016/j.anaerobe.2009.08.002. View

Toure R, Kheadr E, Lacroix C, Moroni O, Fliss I . Production of antibacterial substances by bifidobacterial isolates from infant stool active against Listeria monocytogenes. J Appl Microbiol. 2003; 95(5):1058-69. DOI: 10.1046/j.1365-2672.2003.02085.x. View

Gilliland S, Staley T, BUSH L . Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J Dairy Sci. 1984; 67(12):3045-51. DOI: 10.3168/jds.S0022-0302(84)81670-7. View

Hernandez D, Cardell E, Zarate V . Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711. J Appl Microbiol. 2005; 99(1):77-84. DOI: 10.1111/j.1365-2672.2005.02576.x. View

Savage D . Growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse. Appl Environ Microbiol. 1992; 58(6):1992-5. PMC: 195715. DOI: 10.1128/aem.58.6.1992-1995.1992. View

Sherman P, Ossa J, Johnson-Henry K . Unraveling mechanisms of action of probiotics. Nutr Clin Pract. 2009; 24(1):10-4. DOI: 10.1177/0884533608329231. View

Lin W, Hwang C, Chen L, Tsen H . Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 2006; 23(1):74-81. DOI: 10.1016/j.fm.2005.01.013. View

10.

Cesena C, Morelli L, Alander M, Siljander T, Tuomola E, Salminen S . Lactobacillus crispatus and its nonaggregating mutant in human colonization trials. J Dairy Sci. 2001; 84(5):1001-10. DOI: 10.3168/jds.S0022-0302(01)74559-6. View

11.

Lebeer S, Vanderleyden J, de Keersmaecker S . Genes and molecules of lactobacilli supporting probiotic action. Microbiol Mol Biol Rev. 2008; 72(4):728-64, Table of Contents. PMC: 2593565. DOI: 10.1128/MMBR.00017-08. View

12.

Tareb R, Bernardeau M, Gueguen M, Vernoux J . In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni. J Med Microbiol. 2013; 62(Pt 4):637-649. DOI: 10.1099/jmm.0.049965-0. View

13.

. Technical guidance - Update of the criteria used in the assessment of bacterial resistance to antibiotics of human or veterinary importance. EFSA J. 2023; 6(7):732. PMC: 10193621. DOI: 10.2903/j.efsa.2008.732. View

14.

Ng S, Hart A, Kamm M, Stagg A, Knight S . Mechanisms of action of probiotics: recent advances. Inflamm Bowel Dis. 2008; 15(2):300-10. DOI: 10.1002/ibd.20602. View

15.

Gu R, Yang Z, Li Z, Chen S, Luo Z . Probiotic properties of lactic acid bacteria isolated from stool samples of longevous people in regions of Hotan, Xinjiang and Bama, Guangxi, China. Anaerobe. 2008; 14(6):313-7. DOI: 10.1016/j.anaerobe.2008.06.001. View

16.

Gareau M, Wine E, Rodrigues D, Cho J, Whary M, Philpott D . Bacterial infection causes stress-induced memory dysfunction in mice. Gut. 2010; 60(3):307-17. DOI: 10.1136/gut.2009.202515. View

17.

Miele E, Pascarella F, Giannetti E, Quaglietta L, Baldassano R, Staiano A . Effect of a probiotic preparation (VSL#3) on induction and maintenance of remission in children with ulcerative colitis. Am J Gastroenterol. 2009; 104(2):437-43. DOI: 10.1038/ajg.2008.118. View

18.

Niedzielin K, Kordecki H, Birkenfeld B . A controlled, double-blind, randomized study on the efficacy of Lactobacillus plantarum 299V in patients with irritable bowel syndrome. Eur J Gastroenterol Hepatol. 2001; 13(10):1143-7. DOI: 10.1097/00042737-200110000-00004. View