The E3 Ubiquitin Ligase TaGW2 Facilitates TaSnRK1γ and TaVPS24 Degradation to Enhance Stripe Rust Susceptibility in Wheat

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2024 Dec 3

PMID 39625738

Authors

Shumin Li

Tian Li

Peiyin Zhang

Xuemin Wang

Wenxuan Feng

Yifang Zhang

Bin Chen

Yuling Liu

Gangming Zhan

Chenyang Hao

Xueyong Zhang

Zhensheng Kang

Hude Mao

Affiliations

Soon will be listed here.

Abstract

Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), threatens global wheat production, and therefore discovering genes involved in stripe rust susceptibility is essential for balancing yield with disease resistance in sustainable breeding strategies. Although TaGW2 is well known to negatively regulate wheat kernel size and weight, its role in stress response remains unclear. Here, we found that TaGW2 transcription levels increased following inoculation with Pst or treatment with flg22 or chitin. TaGW2 knockdown lines showed enhanced resistance to multiple Pst races, while TaGW2 overexpression reduced host defence response, promoted Pst growth and development and increased wheat susceptibility to Pst. Additionally, TaGW2 could mediate the ubiquitination and degradation of both TaSnRK1γ and TaVPS24 via the 26S proteasome pathway. Silencing TaSnRK1γ or TaVPS24 in wheat increased sensitivity to Pst, whereas overexpressing either gene enhanced wheat defence response, indicating that TaSnRK1γ and TaVPS24 act as positive regulators of Pst resistance. This study reveals a previously unrecognized mechanism inhibiting plant immunity to Pst through TaGW2-mediated ubiquitination and degradation of TaSnRK1γ and TaVPS24. This work also provides crucial genetic resources for breeding high-yield, stripe rust-resistant wheat varieties.

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