Use of 51Cr Release to Measure the Cytotoxic Effects of Staphylococcal Leukocidin and Toxin Neutralization on Bovine Leukocytes

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1986 Mar 1

PMID 3958139

Citations 3

Authors

D A Loeffler

K A Schat

N L Norcross

Affiliations

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Abstract

Leukocidin toxin from Staphylococcus aureus produces specific cytolytic effects on neutrophils and macrophages. The most commonly used method for determination of leukocidin activity is microscopic examination for characteristic morphological changes in toxin-treated cells. The 51Cr release assay was modified to allow quantitation of the cytolytic effects of leukocidin on bovine peripheral blood neutrophils and lymphocytes. Toxin neutralization by serum and milk samples was quantitated by this method. The neutralizing abilities of the various samples were found to correlate with the levels of immunoglobulin G (IgG1) specific for leukocidin. Undiluted normal serum samples, however, were capable of partially preventing the cytotoxic effects of leukocidin. The assay was shown to be an effective means of quantitating the cytotoxic activity of leukocidin on neutrophils as well as demonstrating neutralization of cytotoxicity by milk and serum samples.

Citing Articles

Leucotoxic activities of Staphylococcus aureus strains isolated from cows, ewes, and goats with mastitis: importance of LukM/LukF'-PV leukotoxin.

Rainard P, Corrales J, Barrio M, Cochard T, Poutrel B Clin Diagn Lab Immunol. 2003; 10(2):272-7.

PMID: 12626454 PMC: 150537. DOI: 10.1128/cdli.10.2.272-277.2003.

Sequencing of leucocidin R from Staphylococcus aureus P83 suggests that staphylococcal leucocidins and gamma-hemolysin are members of a single, two-component family of toxins.

Supersac G, Prevost G, Piemont Y Infect Immun. 1993; 61(2):580-7.

PMID: 8423088 PMC: 302767. DOI: 10.1128/iai.61.2.580-587.1993.

Enzyme-linked immunosorbent assay for detection of leukocidin toxin from Staphylococcus aureus in bovine milk samples.

Loeffler D, Creasy M, Norcross N, Paape M J Clin Microbiol. 1988; 26(7):1331-4.

PMID: 3410947 PMC: 266603. DOI: 10.1128/jcm.26.7.1331-1334.1988.

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