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[Cytotoxic Effects of a Leukocidin from Staphylococcus Aureus (author's Transl)]

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Date 1975 Oct 1
PMID 56107
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Abstract

In an attempt to improve the assay of leukocidin from Staphylococcus aureus a combined vital fluorochrome staining procedure with acridine organe and ethidium bromide was developed. It proved to be more suitable for the demonstration of leukocidic effects on leukocytes than phasecontrastmicroscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes green. With the fluorochrome-method leukocidic changes became also evident in some lymphocytes. These were not clearly demonstrated by phasecontrastmicroscopy.

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Use of 51Cr release to measure the cytotoxic effects of staphylococcal leukocidin and toxin neutralization on bovine leukocytes.

Loeffler D, Schat K, Norcross N J Clin Microbiol. 1986; 23(3):416-20.

PMID: 3958139 PMC: 268665. DOI: 10.1128/jcm.23.3.416-420.1986.