An Analysis of Class I Antigens of Man and Other Species by One-dimensional IEF and Immunoblotting

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1986 Jan 1

PMID 3957414

Citations 21

Authors

J J Neefjes

I Doxiadis

N J Stam

C J Beckers

H L Ploegh

Affiliations

Soon will be listed here.

Abstract

A procedure for the molecular identification of MHC class I products based on 1-D IEF and subsequent immunoblotting is described. Optimal conditions for 1-D IEF, the electrophoretic transfer of proteins out of denaturing, nonionic detergent-containing gels to nitrocellulose, and the requisite antibodies, both polyclonal and monoclonal, for the visualization of class I heavy chains have been established. Cross-reactivity of antibodies has enabled the biochemical analysis of class I heavy chains in the dog. The procedure reported here requires modest amounts of cells and allows a rapid molecular characterization of class I heavy chain polymorphisms in man and other species without the need for radiochemical methods.

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