Derivation of Induced Pluripotent Stem Cell Lines from New Zealand Donors

Overview

Journal J R Soc N Z

Date 2024 Oct 23

PMID 39440007

Authors

Jin Kyo Oh

Aneta Przepiorski

Hao-Han Chang

Rachel C Dodd

Veronika Sander

Brie Sorrenson

Jen-Hsing Shih

Jennifer A Hollywood

Janak R de Zoysa

Peter R Shepherd

Alan J Davidson

Teresa M Holm

Affiliations

Soon will be listed here.

Abstract

We aimed to generate human induced pluripotent stem cell (iPSC) lines from New Zealand donors. These lines are the first to be generated in New Zealand. Human dermal fibroblasts were collected from two individual donors and reprogrammed with the human OSKM transcription factors using the Sendai virus system. Emerging iPSC colonies were picked, expanded and karyotyped. Clones with normal karyotype were characterised for pluripotency marker expression, p53 mutational status and trilineage differentiation potential. The MANZ-2-2 and MANZ-4-37 iPSC lines showed normal karyotype and expressed pluripotency markers at RNA and protein levels without detectable transgene expression. Both lines differentiated into the three germ layers and passed the hPSC Scorecard assay for pluripotency and trilineage differentiation. Furthermore, both lines were susceptible to cell apoptosis mediated by nutlin-3a indicative of their wildtype p53 status. This study presents the successful derivation and characterisation of iPSC lines derived from New Zealand donors. These lines will facilitate iPSC-based research in New Zealand and beyond.

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