3D Resolved Mapping of Optical Aberrations in Thick Tissues

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2012 Aug 10

PMID 22876353

Citations 19

Authors

Jun Zeng

Pierre Mahou

Marie-Claire Schanne-Klein

Emmanuel Beaurepaire

Delphine Debarre

Affiliations

Soon will be listed here.

Abstract

We demonstrate a simple method for mapping optical aberrations with 3D resolution within thick samples. The method relies on the local measurement of the variation in image quality with externally applied aberrations. We discuss the accuracy of the method as a function of the signal strength and of the aberration amplitude and we derive the achievable resolution for the resulting measurements. We then report on measured 3D aberration maps in human skin biopsies and mouse brain slices. From these data, we analyse the consequences of tissue structure and refractive index distribution on aberrations and imaging depth in normal and cleared tissue samples. The aberration maps allow the estimation of the typical aplanetism region size over which aberrations can be uniformly corrected. This method and data pave the way towards efficient correction strategies for tissue imaging applications.

Citing Articles

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