The Lifespan and Kinetics of Human Dendritic Cell Subsets and Their Precursors in Health and Inflammation

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2024 Oct 17

PMID 39417994

Authors

Ruth Lubin

Amit A Patel

Jonas Mackerodt

Yan Zhang

Rotem Gvili

Kevin Mulder

Charles-Antoine Dutertre

Parinaaz Jalali

James R W Glanville

Idit Hazan

Nikhila Sridharan

Gurion Rivkin

Ayse Akarca

Teresa Marafioti

Derek W Gilroy

Leonid Kandel

Alexander Mildner

Asaf Wilensky

Becca Asquith

Florent Ginhoux

Derek Macallan

Simon Yona

Affiliations

Soon will be listed here.

Abstract

Dendritic cells (DC) are specialized mononuclear phagocytes that link innate and adaptive immunity. They comprise two principal subsets: plasmacytoid DC (pDC) and conventional DC (cDC). Understanding the generation, differentiation, and migration of cDC is critical for immune homeostasis. Through human in vivo deuterium-glucose labeling, we observed the rapid appearance of AXL+ Siglec6+ DC (ASDC) in the bloodstream. ASDC circulate for ∼2.16 days, while cDC1 and DC2 circulate for ∼1.32 and ∼2.20 days, respectively, upon release from the bone marrow. Interestingly, DC3, a cDC subset that shares several similarities with monocytes, exhibits a labeling profile closely resembling that of DC2. In a human in vivo model of cutaneous inflammation, ASDC were recruited to the inflammatory site, displaying a distinctive effector signature. Taken together, these results quantify the ephemeral circulating lifespan of human cDC and propose functions of cDC and their precursors that are rapidly recruited to sites of inflammation.

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