Mitochondria: the Beating Heart of the Eukaryotic Cell

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2024 Oct 4

PMID 39367527

Authors

Johannes M Herrmann

Affiliations

Soon will be listed here.

Abstract

Mitochondria are essential organelles of eukaryotic cells. They consist of hundreds of proteins, which are synthesized in the cytosol and imported into mitochondria via different targeting routes. In addition, a small number of proteins are encoded by the organellar genome and synthesized by mitochondrial ribosomes. In this 'In the Limelight' special issue of FEBS Open Bio, five review articles describe these different biogenesis routes of mitochondrial proteins and provide a comprehensive overview of the structures and mechanisms by which mitochondrial proteins are synthesized and transported to their respective location within the organelle. These reviews, written by leading experts, provide a general overview, but also highlight current developments in the field of mitochondrial biogenesis.

References

Hohr A, Lindau C, Wirth C, Qiu J, Stroud D, Kutik S . Membrane protein insertion through a mitochondrial β-barrel gate. Science. 2018; 359(6373). PMC: 5959003. DOI: 10.1126/science.aah6834. View

Diederichs K, Ni X, Rollauer S, Botos I, Tan X, King M . Structural insight into mitochondrial β-barrel outer membrane protein biogenesis. Nat Commun. 2020; 11(1):3290. PMC: 7335169. DOI: 10.1038/s41467-020-17144-1. View

Morgenstern M, Stiller S, Lubbert P, Peikert C, Dannenmaier S, Drepper F . Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale. Cell Rep. 2017; 19(13):2836-2852. PMC: 5494306. DOI: 10.1016/j.celrep.2017.06.014. View

Sim S, Chen Y, Lynch D, Gumbart J, Park E . Structural basis of mitochondrial protein import by the TIM23 complex. Nature. 2023; 621(7979):620-626. PMC: 11495887. DOI: 10.1038/s41586-023-06239-6. View

Takeda H, Tsutsumi A, Nishizawa T, Lindau C, Busto J, Wenz L . Mitochondrial sorting and assembly machinery operates by β-barrel switching. Nature. 2021; 590(7844):163-169. DOI: 10.1038/s41586-020-03113-7. View

Kizmaz B, Nutz A, Egeler A, Herrmann J . Protein insertion into the inner membrane of mitochondria: routes and mechanisms. FEBS Open Bio. 2024; 14(10):1627-1639. PMC: 11452304. DOI: 10.1002/2211-5463.13806. View

Zhou X, Yang Y, Wang G, Wang S, Sun D, Ou X . Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex. Nat Struct Mol Biol. 2023; 30(12):1996-2008. DOI: 10.1038/s41594-023-01103-7. View

Maruszczak K, Draczkowski P, Wnorowski A, Chacinska A . Structure prediction analysis of human core TIM23 complex reveals conservation of the protein translocation mechanism. FEBS Open Bio. 2024; 14(10):1656-1667. PMC: 11452300. DOI: 10.1002/2211-5463.13840. View

Zarges C, Riemer J . Oxidative protein folding in the intermembrane space of human mitochondria. FEBS Open Bio. 2024; 14(10):1610-1626. PMC: 11452306. DOI: 10.1002/2211-5463.13839. View

10.

Takeda H, Busto J, Lindau C, Tsutsumi A, Tomii K, Imai K . A multipoint guidance mechanism for β-barrel folding on the SAM complex. Nat Struct Mol Biol. 2023; 30(2):176-187. DOI: 10.1038/s41594-022-00897-2. View

11.

Pagliarini D, Rutter J . Hallmarks of a new era in mitochondrial biochemistry. Genes Dev. 2013; 27(24):2615-27. PMC: 3877752. DOI: 10.1101/gad.229724.113. View

12.

Lill R . From the discovery to molecular understanding of cellular iron-sulfur protein biogenesis. Biol Chem. 2020; 401(6-7):855-876. DOI: 10.1515/hsz-2020-0117. View

13.

Ott M, Amunts A, Brown A . Organization and Regulation of Mitochondrial Protein Synthesis. Annu Rev Biochem. 2016; 85:77-101. DOI: 10.1146/annurev-biochem-060815-014334. View

14.

Ganesan I, Busto J, Pfanner N, Wiedemann N . Biogenesis of mitochondrial β-barrel membrane proteins. FEBS Open Bio. 2024; 14(10):1595-1609. PMC: 11452307. DOI: 10.1002/2211-5463.13905. View

15.

Fielden L, Busch J, Merkt S, Ganesan I, Steiert C, Hasselblatt H . Central role of Tim17 in mitochondrial presequence protein translocation. Nature. 2023; 621(7979):627-634. PMC: 10511324. DOI: 10.1038/s41586-023-06477-8. View

16.

Rath S, Sharma R, Gupta R, Ast T, Chan C, Durham T . MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations. Nucleic Acids Res. 2020; 49(D1):D1541-D1547. PMC: 7778944. DOI: 10.1093/nar/gkaa1011. View

17.

Brischigliaro M, Sierra-Magro A, Ahn A, Barrientos A . Mitochondrial ribosome biogenesis and redox sensing. FEBS Open Bio. 2024; 14(10):1640-1655. PMC: 11452305. DOI: 10.1002/2211-5463.13844. View