MitoCarta3.0: an Updated Mitochondrial Proteome Now with Sub-organelle Localization and Pathway Annotations

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2020 Nov 11

PMID 33174596

Citations 607

Authors

Sneha Rath

Rohit Sharma

Rahul Gupta

Tslil Ast

Connie Chan

Timothy J Durham

Russell P Goodman

Zenon Grabarek

Mary E Haas

Wendy H W Hung

Pallavi R Joshi

Alexis A Jourdain

Sharon H Kim

Anna V Kotrys

Stephanie S Lam

Jason G McCoy

Joshua D Meisel

Maria Miranda

Apekshya Panda

Anupam Patgiri

Robert Rogers

Shayan Sadre

Hardik Shah

Owen S Skinner

Tsz-Leung To

Melissa A Walker

Hong Wang

Patrick S Ward

Jordan Wengrod

Chen-Ching Yuan

Sarah E Calvo

Vamsi K Mootha

Affiliations

Soon will be listed here.

Abstract

The mammalian mitochondrial proteome is under dual genomic control, with 99% of proteins encoded by the nuclear genome and 13 originating from the mitochondrial DNA (mtDNA). We previously developed MitoCarta, a catalogue of over 1000 genes encoding the mammalian mitochondrial proteome. This catalogue was compiled using a Bayesian integration of multiple sequence features and experimental datasets, notably protein mass spectrometry of mitochondria isolated from fourteen murine tissues. Here, we introduce MitoCarta3.0. Beginning with the MitoCarta2.0 inventory, we performed manual review to remove 100 genes and introduce 78 additional genes, arriving at an updated inventory of 1136 human genes. We now include manually curated annotations of sub-mitochondrial localization (matrix, inner membrane, intermembrane space, outer membrane) as well as assignment to 149 hierarchical 'MitoPathways' spanning seven broad functional categories relevant to mitochondria. MitoCarta3.0, including sub-mitochondrial localization and MitoPathway annotations, is freely available at http://www.broadinstitute.org/mitocarta and should serve as a continued community resource for mitochondrial biology and medicine.

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