Resolving the Structure of a Guanine Quadruplex in TMPRSS2 Messenger RNA by Circular Dichroism and Molecular Modeling

Overview

Journal Chemistry

Specialty Chemistry

Date 2024 Oct 4

PMID 39365977

Authors

Luisa DAnna

Aurane Froux

Aurianne Rainot

Angelo Spinello

Ugo Perricone

Florent Barbault

Stephanie Grandemange

Giampaolo Barone

Alessio Terenzi

Antonio Monari

Affiliations

Soon will be listed here.

Abstract

The presence of a guanine quadruplex in the opening reading frame of the messenger RNA coding for the transmembrane serine protease 2 (TMPRSS2) may pave the way to original anticancer and host-oriented antiviral strategy. Indeed, TMPRSS2 in addition to being overexpressed in different cancer types, is also related to the infection of respiratory viruses, including SARS-CoV-2, by promoting the cellular and viral membrane fusion through its proteolytic activity. The design of selective ligands targeting TMPRSS2 messenger RNA requires a detailed knowledge, at atomic level, of its structure. Therefore, we have used an original experimental-computational protocol to predict the first resolved structure of the parallel guanine quadruplex secondary structure in the RNA of TMPRSS2, which shows a rigid core flanked by a flexible loop. This represents the first atomic scale structure of the guanine quadruplex structure present in TMPRSS2 messenger RNA.

Citing Articles

Resolving the Structure of a Guanine Quadruplex in TMPRSS2 Messenger RNA by Circular Dichroism and Molecular Modeling.

DAnna L, Froux A, Rainot A, Spinello A, Perricone U, Barbault F Chemistry. 2024; 30(71):e202403572.

PMID: 39365977 PMC: 11653239. DOI: 10.1002/chem.202403572.

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