Effect of Cell-free Culture on Bacterial Pathogens Isolated from Cystic Fibrosis Patients: and Studies

This study aimed to investigate the effects of the cell-free supernatant of ATCC 10241 on the biofilm-forming capacity of strains isolated from cystic fibrosis (CF) patients. In addition, the study evaluated the potential of the cell-free supernatant to modulate inflammation and reduce lung damage in mice infected with strains or co-challenged with and the group (SMG). The results showed that CF-derived strains can infect the respiratory tract of adult mice, inducing local inflammation and lung damage. The severity of these infections was exacerbated when was co-administered with SMG. Notably, nebulization with the cell-free supernatant of produced beneficial effects, reducing respiratory infection severity and inflammatory responses induced by , both alone or in combination with SMG. Reduced bacterial loads and lung damage were observed in supernatant-treated mice compared to controls. Although further mechanistic studies are necessary, the results show that the cell-free supernatant of ATCC 10241 is an interesting adjuvant alternative to treat respiratory infections and superinfections in CF patients.