Genetically Diverse Pseudomonas Aeruginosa Populations Display Similar Transcriptomic Profiles in a Cystic Fibrosis Explanted Lung

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Aug 1

PMID 31363089

Citations 45

Authors

Adrian Kordes

Matthias Preusse

Sven D Willger

Peter Braubach

Danny Jonigk

Axel Haverich

Gregor Warnecke

Susanne Haussler

Affiliations

Soon will be listed here.

Abstract

Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic variation, the ex vivo transcriptional profiles of P. aeruginosa populations inhabiting different regions of the CF lung are similar. Although we cannot estimate the extent to which the transcriptional response recorded here actually reflects the in vivo transcriptomes, our results indicate that there may be a common in vivo transcriptional profile in the CF lung environment.

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