Preventing SARS-CoV-2 Infection Using Fv-antibodies Targeting the Proprotein Convertase (PPC) Cleavage Site

Overview

Journal RSC Med Chem

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Sep 18

PMID 39290379

Authors

Jaeyong Jung

Jeong Soo Sung

Soonil Kwon

Hyung Eun Bae

Min-Jung Kang

Joachim Jose

Misu Lee

Jae-Chul Pyun

Affiliations

Soon will be listed here.

Abstract

Fv-antibodies targeting the proprotein convertase (PPC) region of the SARS-CoV-2 spike protein (SP) were screened from an Fv-antibody library to inhibit SARS-CoV-2 infection. Two selected Fv-antibodies were expressed as soluble recombinant proteins, and their binding affinities were assessed using a surface plasmon resonance biosensor. The binding regions of these Fv-antibodies corresponded to the cleavage sites of furin (S1/S2) and transmembrane serine protease 2 (TMPRSS2, S2'). The neutralizing activities of the two Fv-antibodies were demonstrated using a cell-based infection assay with pseudo-viruses carrying the SP of four different SARS-CoV-2 variants: wild-type (D614), delta (B.1.617.2), omicron (BA.2), and omicron (BA.4/5).

Citing Articles

Transmembrane protease serine 2 (TMPRSS2) inhibitors screened from an Fv-antibody library for preventing SARS-CoV-2 infection.

Jung J, Sung J, Kwon S, Bae H, Kang M, Jose J RSC Med Chem. 2025; .

PMID: 39990164 PMC: 11843257. DOI: 10.1039/d4md00992d.

Screened Fv-Antibodies against the Angiotensin-Converting Enzyme 2 (ACE2) Receptor Neutralizing the Infection of SARS-CoV-2.

Jung J, Kwon S, Sung J, Bae H, Kang M, Jose J ACS Pharmacol Transl Sci. 2024; 7(12):3914-3920.

PMID: 39698273 PMC: 11651164. DOI: 10.1021/acsptsci.4c00441.

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