YY1-controlled Regulatory Connectivity and Transcription Are Influenced by the Cell Cycle

Overview

Journal Nat Genet

Specialty Genetics

Date 2024 Aug 29

PMID 39210046

Authors

Jessica C Lam

Nicholas G Aboreden

Susannah C Midla

Siqing Wang

Anran Huang

Cheryl A Keller

Belinda Giardine

Kate A Henderson

Ross C Hardison

Haoyue Zhang

Gerd A Blobel

Affiliations

Soon will be listed here.

Abstract

Few transcription factors have been examined for their direct roles in physically connecting enhancers and promoters. Here acute degradation of Yin Yang 1 (YY1) in erythroid cells revealed its requirement for the maintenance of numerous enhancer-promoter loops, but not compartments or domains. Despite its reported ability to interact with cohesin, the formation of YY1-dependent enhancer-promoter loops does not involve stalling of cohesin-mediated loop extrusion. Integrating mitosis-to-G1-phase dynamics, we observed partial retention of YY1 on mitotic chromatin, predominantly at gene promoters, followed by rapid rebinding during mitotic exit, coinciding with enhancer-promoter loop establishment. YY1 degradation during the mitosis-to-G1-phase interval revealed a set of enhancer-promoter loops that require YY1 for establishment during G1-phase entry but not for maintenance in interphase, suggesting that cell cycle stage influences YY1's architectural function. Thus, as revealed here for YY1, chromatin architectural functions of transcription factors can vary in their interplay with CTCF and cohesin as well as by cell cycle stage.

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