Physiological Significance of the Two Isoforms of Initiator TRNAs in

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2024 Aug 22

PMID 39171914

Authors

Amit Kumar Sahu

Riyaz Ahmad Shah

Divya Nashier

Prafful Sharma

Rajagopal Varada

Kuldeep Lahry

Sudhir Singh

Sunil Shetty

Tanweer Hussain

Umesh Varshney

Affiliations

Soon will be listed here.

Abstract

Importance: harbors four initiator tRNA (i-tRNA) genes: three of these at and the fourth one at loci. In B, all four genes encode i-tRNA. In K, because of a single-nucleotide polymorphism, metY encodes a variant, i-tRNA, having an A in place of G at position 46 of i-tRNA sequence in metY. We show that G46 confers stability to i-tRNA. The strains sustained on i-tRNA have a growth fitness advantage over those sustained on i-tRNA. Strains harboring (B mimic) or (K mimic) show that while in the nutrient-rich media, the K mimic is outcompeted rapidly; in the nutrient-poor medium, the K mimic is outcompeted less rapidly.

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