Primer Exchange Reaction Assisted CRISPR/Cas9 Cleavage for Detection of Dual MicroRNAs with Electrochemistry Method

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2024 Aug 2

PMID 39093358

Authors

Yanmei Zhou

Shengjun Che

Zhili Wang

Xiaoru Zhang

Xunyi Yuan

Affiliations

Soon will be listed here.

Abstract

An electrochemical sensor assisted by primer exchange reaction (PER) and CRISPR/Cas9 system (PER-CRISPR/Cas9-E) was established for the sensitive detection of dual microRNAs (miRNAs). Two PER hairpin (HP) were designed to produce a lot of extended PER products, which could hybridize with two kinds of hairpin probes modified on the electrode and initiate the cleavage of two CRISPR/Cas9 systems guided by single guide RNAs (sgRNAs) with different recognition sequences. The decrease of the two electrochemical redox signals indicated the presence of dual-target miRNAs. With the robustness and high specificity of PER amplification and CRISPR/Cas9 cleavage system, simultaneous detection of two targets was achieved and the detection limits for miRNA-21 and miRNA-155 were 0.43 fM and 0.12 fM, respectively. The developed biosensor has the advantages of low cost, easy operation, and in-situ detection, providing a promising platform for point-of-care detection of multiple miRNAs.

Citing Articles

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PMID: 39797940 DOI: 10.1007/s11033-024-10205-4.

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