A Novel MAP Kinase-interacting Protein MoSmi1 Regulates Development and Pathogenicity in Magnaporthe Oryzae

Overview

Journal Mol Plant Pathol

Specialty Molecular Biology

Date 2024 Jul 22

PMID 39034619

Authors

Yu Wang

Xinyue Cui

Junlian Xiao

Xiaoru Kang

Jinmei Hu

Zhicheng Huang

Na Li

Chuyu Yang

Yuemin Pan

Shulin Zhang

Affiliations

Soon will be listed here.

Abstract

The cell wall is the first barrier against external adversity and plays roles in maintaining normal physiological functions of fungi. Previously, we reported a nucleosome assembly protein, MoNap1, in Magnaporthe oryzae that plays a role in cell wall integrity (CWI), stress response, and pathogenicity. Moreover, MoNap1 negatively regulates the expression of MoSMI1 encoded by MGG_03970. Here, we demonstrated that deletion of MoSMI1 resulted in a significant defect in appressorium function, CWI, cell morphology, and pathogenicity. Further investigation revealed that MoSmi1 interacted with MoOsm1 and MoMps1 and affected the phosphorylation levels of MoOsm1, MoMps1, and MoPmk1, suggesting that MoSmi1 regulates biological functions by mediating mitogen-activated protein kinase (MAPK) signalling pathway in M. oryzae. In addition, transcriptome data revealed that MoSmi1 regulates many infection-related processes in M. oryzae, such as membrane-related pathway and oxidation reduction process. In conclusion, our study demonstrated that MoSmi1 regulates CWI by mediating the MAPK pathway to affect development and pathogenicity of M. oryzae.

Citing Articles

A novel MAP kinase-interacting protein MoSmi1 regulates development and pathogenicity in Magnaporthe oryzae.

Wang Y, Cui X, Xiao J, Kang X, Hu J, Huang Z Mol Plant Pathol. 2024; 25(7):e13493.

PMID: 39034619 PMC: 11260997. DOI: 10.1111/mpp.13493.

References

Bohnert S, Neumann H, Thines E, Jacob S . Visualizing fungicide action: an in vivo tool for rapid validation of fungicides with target location HOG pathway. Pest Manag Sci. 2018; 75(3):772-778. DOI: 10.1002/ps.5177. View

Ebbole D . Magnaporthe as a model for understanding host-pathogen interactions. Annu Rev Phytopathol. 2007; 45:437-56. DOI: 10.1146/annurev.phyto.45.062806.094346. View

Li G, Zhang X, Tian H, Choi Y, Tao W, Xu J . MST50 is involved in multiple MAP kinase signaling pathways in Magnaporthe oryzae. Environ Microbiol. 2017; 19(5):1959-1974. DOI: 10.1111/1462-2920.13710. View

Steiner A, Rybak K, Altmann M, McFarlane H, Klaeger S, Nguyen N . Cell cycle-regulated PLEIADE/AtMAP65-3 links membrane and microtubule dynamics during plant cytokinesis. Plant J. 2016; 88(4):531-541. DOI: 10.1111/tpj.13275. View

Dunker A, Cortese M, Romero P, Iakoucheva L, Uversky V . Flexible nets. The roles of intrinsic disorder in protein interaction networks. FEBS J. 2005; 272(20):5129-48. DOI: 10.1111/j.1742-4658.2005.04948.x. View

Abenza J, Pantazopoulou A, Rodriguez J, Galindo A, Penalva M . Long-distance movement of Aspergillus nidulans early endosomes on microtubule tracks. Traffic. 2008; 10(1):57-75. DOI: 10.1111/j.1600-0854.2008.00848.x. View

Kuravi V, Kurischko C, Puri M, Luca F . Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock. Mol Biol Cell. 2011; 22(24):4892-907. PMC: 3237631. DOI: 10.1091/mbc.E11-04-0371. View

Orlean P . Architecture and biosynthesis of the Saccharomyces cerevisiae cell wall. Genetics. 2012; 192(3):775-818. PMC: 3522159. DOI: 10.1534/genetics.112.144485. View

Yi M, Chi M, Khang C, Park S, Kang S, Valent B . The ER chaperone LHS1 is involved in asexual development and rice infection by the blast fungus Magnaporthe oryzae. Plant Cell. 2009; 21(2):681-95. PMC: 2660637. DOI: 10.1105/tpc.107.055988. View

10.

Manning G, Whyte D, Martinez R, Hunter T, Sudarsanam S . The protein kinase complement of the human genome. Science. 2002; 298(5600):1912-34. DOI: 10.1126/science.1075762. View

11.

Hughes J, Meireles A, Fisher K, Garcia A, Antrobus P, Wainman A . A microtubule interactome: complexes with roles in cell cycle and mitosis. PLoS Biol. 2008; 6(4):e98. PMC: 2323305. DOI: 10.1371/journal.pbio.0060098. View

12.

Saito M, Hess D, Eglinger J, Fritsch A, Kreysing M, Weinert B . Acetylation of intrinsically disordered regions regulates phase separation. Nat Chem Biol. 2018; 15(1):51-61. DOI: 10.1038/s41589-018-0180-7. View

13.

Bellingham-Johnstun K, Thorn A, Belmonte J, Laplante C . Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast. Mol Biol Cell. 2023; 34(8):ar77. PMC: 10398876. DOI: 10.1091/mbc.E23-01-0034. View

14.

Uversky V . A decade and a half of protein intrinsic disorder: biology still waits for physics. Protein Sci. 2013; 22(6):693-724. PMC: 3690711. DOI: 10.1002/pro.2261. View

15.

Martin-Yken H, Dagkessamanskaia A, Basmaji F, Lagorce A, Francois J . The interaction of Slt2 MAP kinase with Knr4 is necessary for signalling through the cell wall integrity pathway in Saccharomyces cerevisiae. Mol Microbiol. 2003; 49(1):23-35. DOI: 10.1046/j.1365-2958.2003.03541.x. View

16.

Majumdar A, Dogra P, Maity S, Mukhopadhyay S . Liquid-Liquid Phase Separation Is Driven by Large-Scale Conformational Unwinding and Fluctuations of Intrinsically Disordered Protein Molecules. J Phys Chem Lett. 2019; 10(14):3929-3936. DOI: 10.1021/acs.jpclett.9b01731. View

17.

Xu J, Staiger C, Hamer J . Inactivation of the mitogen-activated protein kinase Mps1 from the rice blast fungus prevents penetration of host cells but allows activation of plant defense responses. Proc Natl Acad Sci U S A. 1998; 95(21):12713-8. PMC: 22896. DOI: 10.1073/pnas.95.21.12713. View

18.

Yu G, Wang L, Han Y, He Q . clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS. 2012; 16(5):284-7. PMC: 3339379. DOI: 10.1089/omi.2011.0118. View

19.

Xu J, Hamer J . MAP kinase and cAMP signaling regulate infection structure formation and pathogenic growth in the rice blast fungus Magnaporthe grisea. Genes Dev. 1996; 10(21):2696-706. DOI: 10.1101/gad.10.21.2696. View

20.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View