A Chemoproteomic Approach to Monitor Native Iron-Sulfur Cluster Binding

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jul 15

PMID 39008260

Authors

Daniel W Bak

Eranthie Weerapana

Affiliations

Soon will be listed here.

Abstract

Iron-sulfur (Fe-S) clusters are essential redox-active metallocofactors participating in electron transfer, radical chemistry, primary metabolism, and gene regulation. Successful trafficking and incorporation of Fe-S clusters into target proteins are critical to proper cellular function. While biophysical studies of isolated Fe-S proteins provide insight into the structure and function of these inorganic cofactors, few strategies currently exist to directly interrogate Fe-S cluster binding within a cellular environment. Here, we describe a chemoproteomic platform to report on Fe-S cluster incorporation and occupancy directly within a native proteome, enabling the characterization of Fe-S biogenesis pathways and the identification of undiscovered Fe-S proteins.

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