Cloning, Sequence, and Expression of a Human Granulocyte/macrophage Colony-stimulating Factor

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Sep 1

PMID 3898082

Citations 54

Authors

M A Cantrell

D Anderson

D P Cerretti

V Price

K McKereghan

R J Tushinski

D Y Mochizuki

A Larsen

K Grabstein

S Gillis

Affiliations

Soon will be listed here.

Abstract

Human granulocyte/macrophage colony-stimulating factor (GM-CSF) is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. In this report we have used a mouse GM-CSF cDNA clone to isolate human GM-CSF clones from libraries made from HUT-102 messenger RNA and mitogen-stimulated T-lymphocyte messenger RNA. The human cDNA clones contained a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and showed 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. One of these cDNA clones was shown to direct the synthesis of biologically active GM-CSF using a yeast expression system. The gene for human GM-CSF appears to exist as a single-copy gene.

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