Alpha-factor-directed Synthesis and Secretion of Mature Foreign Proteins in Saccharomyces Cerevisiae

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Aug 1

PMID 6087338

Citations 103

Authors

A J Brake

J P Merryweather

D G Coit

U A Heberlein

F R Masiarz

G T Mullenbach

M S Urdea

P Valenzuela

P J Barr

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

Citing Articles

Efficient expression and purification of rat CRP in .

Cheng B, Tang Y, Gou Y, Li J, Xu T, Zhu L Front Immunol. 2024; 15:1465365.

PMID: 39253072 PMC: 11381232. DOI: 10.3389/fimmu.2024.1465365.

as a Platform for Heterologous Expression of Enzymes Used for Industry.

Khlebodarova T, Bogacheva N, Zadorozhny A, Bryanskaya A, Vasilieva A, Chesnokov D Microorganisms. 2024; 12(2).

PMID: 38399750 PMC: 10892927. DOI: 10.3390/microorganisms12020346.

Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system.

Eskandari A, Nezhad N, Leow T, Rahman M, Oslan S World J Microbiol Biotechnol. 2023; 40(1):39.

PMID: 38062216 DOI: 10.1007/s11274-023-03851-6.

Recombinant Production of Food Allergens in Yeast Pichia pastoris.

Piboonpocanun S Methods Mol Biol. 2023; 2717:29-40.

PMID: 37737976 DOI: 10.1007/978-1-0716-3453-0_3.

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions.

De Groeve M, Laukens B, Schotte P Microb Cell Fact. 2023; 22(1):135.

PMID: 37481525 PMC: 10362571. DOI: 10.1186/s12934-023-02132-z.

References

EDMAN P, Begg G . A protein sequenator. Eur J Biochem. 1967; 1(1):80-91. DOI: 10.1007/978-3-662-25813-2_14. View

Hitzeman R, Leung D, Perry L, Kohr W, Levine H, Goeddel D . Secretion of human interferons by yeast. Science. 1983; 219(4585):620-5. DOI: 10.1126/science.6186023. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Cohen S, Carpenter G . Human epidermal growth factor: isolation and chemical and biological properties. Proc Natl Acad Sci U S A. 1975; 72(4):1317-21. PMC: 432524. DOI: 10.1073/pnas.72.4.1317. View

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Hinnen A, Hicks J, Fink G . Transformation of yeast. Proc Natl Acad Sci U S A. 1978; 75(4):1929-33. PMC: 392455. DOI: 10.1073/pnas.75.4.1929. View

Beggs J . Transformation of yeast by a replicating hybrid plasmid. Nature. 1978; 275(5676):104-9. DOI: 10.1038/275104a0. View

Carpenter G, Cohen S . Epidermal growth factor. Annu Rev Biochem. 1979; 48:193-216. DOI: 10.1146/annurev.bi.48.070179.001205. View

KREIL G, Haiml L, Suchanek G . Stepwise cleavage of the pro part of promelittin by dipeptidylpeptidase IV. Evidence for a new type of precursor--product conversion. Eur J Biochem. 1980; 111(1):49-58. DOI: 10.1111/j.1432-1033.1980.tb06073.x. View

10.

Fasiolo F, Bonnet J, Lacroute F . Cloning of the yeast methionyl-tRNA synthetase gene. J Biol Chem. 1981; 256(5):2324-8. View

11.

Hewick R, Hunkapiller M, HOOD L, Dreyer W . A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981; 256(15):7990-7. View

12.

Ish-Horowicz D, Burke J . Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981; 9(13):2989-98. PMC: 327326. DOI: 10.1093/nar/9.13.2989. View

13.

Novick P, Ferro S, Schekman R . Order of events in the yeast secretory pathway. Cell. 1981; 25(2):461-9. DOI: 10.1016/0092-8674(81)90064-7. View

14.

PERLMAN D, HALVORSON H . Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene. Cell. 1981; 25(2):525-36. DOI: 10.1016/0092-8674(81)90071-4. View

15.

Talmadge K, Brosius J, Gilbert W . An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria. Nature. 1981; 294(5837):176-8. DOI: 10.1038/294176a0. View

16.

Carlson M, Botstein D . Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell. 1982; 28(1):145-54. DOI: 10.1016/0092-8674(82)90384-1. View

17.

Kurjan J, Herskowitz I . Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor. Cell. 1982; 30(3):933-43. DOI: 10.1016/0092-8674(82)90298-7. View

18.

Julius D, Blair L, Brake A, Sprague G, Thorner J . Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Cell. 1983; 32(3):839-52. DOI: 10.1016/0092-8674(83)90070-3. View

19.

Brake A, Julius D, Thorner J . A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence. Mol Cell Biol. 1983; 3(8):1440-50. PMC: 369990. DOI: 10.1128/mcb.3.8.1440-1450.1983. View

20.

Emr S, Schekman R, Flessel M, Thorner J . An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc Natl Acad Sci U S A. 1983; 80(23):7080-4. PMC: 389996. DOI: 10.1073/pnas.80.23.7080. View