Metaproteomics for Coinfections in the Upper Respiratory Tract: The Case of COVID-19

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Jun 28

PMID 38941023

Authors

Surbhi Bihani

Aryan Gupta

Subina Mehta

Andrew Rajczewski

Timothy Griffin

Pratik Jagtap

Sanjeeva Srivastava

Affiliations

Soon will be listed here.

Abstract

The upper respiratory tract (URT) is home to a diverse range of microbial species. Respiratory infections disturb the microbial flora in the URT, putting people at risk of secondary infections. The potential dangers and clinical effects of bacterial and fungal coinfections with SARS-CoV-2 support the need to investigate the microbiome of the URT using clinical samples. Mass spectrometry (MS)-based metaproteomics analysis of microbial proteins is a novel approach to comprehensively assess the clinical specimens with complex microbial makeup. The coronavirus that causes severe acute respiratory syndrome (SARS-CoV-2) is responsible for the COVID-19 pandemic resulting in a plethora of microbial coinfections impeding therapy, prognosis, and overall disease management. In this chapter, the corresponding workflows for MS-based shotgun proteomics and metaproteomic analysis are illustrated.

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