Protein Kinase Activity Associated with the Product of the Yeast Cell Division Cycle Gene CDC28

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Jun 1

PMID 3889921

Citations 138

Authors

S I Reed

J A Hadwiger

A T Lorincz

Affiliations

Soon will be listed here.

Abstract

Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.

Citing Articles

Cadmium binding by the F-box domain induces p97-mediated SCF complex disassembly to activate stress response programs.

Lauinger L, Andronicos A, Flick K, Yu C, Durairaj G, Huang L Nat Commun. 2024; 15(1):3894.

PMID: 38719837 PMC: 11079001. DOI: 10.1038/s41467-024-48184-6.

Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery.

Wang X, Pai C, Stone D J Cell Biol. 2022; 221(12).

PMID: 36156058 PMC: 9516845. DOI: 10.1083/jcb.202203004.

Cdc48 cofactor Shp1 regulates signal-induced SCF disassembly.

Lauinger L, Flick K, Yen J, Mathur R, Kaiser P Proc Natl Acad Sci U S A. 2020; 117(35):21319-21327.

PMID: 32817489 PMC: 7474596. DOI: 10.1073/pnas.1922891117.

Haspin regulates Ras localization to promote Cdc24-driven mitotic depolarization.

Quadri R, Galli M, Galati E, Rotondo G, Gallo G, Panigada D Cell Discov. 2020; 6:42.

PMID: 32595981 PMC: 7308332. DOI: 10.1038/s41421-020-0170-2.

Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine.

Wang X, Tian W, Banh B, Statler B, Liang J, Stone D J Cell Biol. 2019; 218(11):3730-3752.

PMID: 31570500 PMC: 6829655. DOI: 10.1083/jcb.201901155.

References

Ueda Y, Kakimoto S, Oshima Y . Isolation and characterization of acid phosphatase mutants in Saccharomyces cerevisiae. J Bacteriol. 1973; 113(2):727-38. PMC: 285288. DOI: 10.1128/jb.113.2.727-738.1973. View

Hartwell L . Macromolecule synthesis in temperature-sensitive mutants of yeast. J Bacteriol. 1967; 93(5):1662-70. PMC: 276664. DOI: 10.1128/jb.93.5.1662-1670.1967. View

Chou P, Fasman G . Prediction of protein conformation. Biochemistry. 1974; 13(2):222-45. DOI: 10.1021/bi00699a002. View

Collett M, Erikson R . Protein kinase activity associated with the avian sarcoma virus src gene product. Proc Natl Acad Sci U S A. 1978; 75(4):2021-4. PMC: 392475. DOI: 10.1073/pnas.75.4.2021. View

Scherer S, Davis R . Replacement of chromosome segments with altered DNA sequences constructed in vitro. Proc Natl Acad Sci U S A. 1979; 76(10):4951-5. PMC: 413056. DOI: 10.1073/pnas.76.10.4951. View

Hunter T, Sefton B . Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc Natl Acad Sci U S A. 1980; 77(3):1311-5. PMC: 348484. DOI: 10.1073/pnas.77.3.1311. View

Reed S . The selection of S. cerevisiae mutants defective in the start event of cell division. Genetics. 1980; 95(3):561-77. PMC: 1214247. DOI: 10.1093/genetics/95.3.561. View

Buhrow S, Cohen S, Staros J . Affinity labeling of the protein kinase associated with the epidermal growth factor receptor in membrane vesicles from A431 cells. J Biol Chem. 1982; 257(8):4019-22. View

Reed S, Ferguson J, Groppe J . Preliminary characterization of the transcriptional and translational products of the Saccharomyces cerevisiae cell division cycle gene CDC28. Mol Cell Biol. 1982; 2(4):412-25. PMC: 369805. DOI: 10.1128/mcb.2.4.412-425.1982. View

10.

Reed S . Preparation of product-specific antisera by gene fusion: antibodies specific for the product of the yeast cell-division-cycle gene CDC28. Gene. 1982; 20(2):255-65. DOI: 10.1016/0378-1119(82)90044-0. View

11.

Kloetzer W, Maxwell S, Arlinghaus R . P85gag-mos encoded by ts110 Moloney murine sarcoma virus has an associated protein kinase activity. Proc Natl Acad Sci U S A. 1983; 80(2):412-6. PMC: 393387. DOI: 10.1073/pnas.80.2.412. View

12.

Lorincz A, Reed S . Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes. Nature. 1984; 307(5947):183-5. DOI: 10.1038/307183a0. View

13.

Papkoff J, Nigg E, Hunter T . The transforming protein of Moloney murine sarcoma virus is a soluble cytoplasmic protein. Cell. 1983; 33(1):161-72. DOI: 10.1016/0092-8674(83)90345-8. View

14.

Moelling K, Heimann B, Beimling P, Rapp U, Sander T . Serine- and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature. 1984; 312(5994):558-61. DOI: 10.1038/312558a0. View

15.

Lorincz A, Miller M, Xuong N, Geiduschek E . Identification of proteins whose synthesis is modulated during the cell cycle of Saccharomyces cerevisiae. Mol Cell Biol. 1982; 2(12):1532-49. PMC: 369962. DOI: 10.1128/mcb.2.12.1532-1549.1982. View

16.

Hartwell L, Culotti J, Pringle J, Reid B . Genetic control of the cell division cycle in yeast. Science. 1974; 183(4120):46-51. DOI: 10.1126/science.183.4120.46. View