The Purification of Shikimate Dehydrogenase from Escherichia Coli

Overview

Journal Biochem J

Specialty Biochemistry

Date 1985 Feb 15

PMID 3883995

Citations 15

Authors

S Chaudhuri

J R Coggins

Affiliations

Soon will be listed here.

Abstract

A procedure was developed for the purification of shikimate dehydrogenase from Escherichia coli. Homogeneous enzyme with specific activity 1100 units/mg of protein was obtained in 21% overall yield. The subunit Mr estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 32 000. The native Mr, estimated by gel-permeation chromatography on a TSK G2000SW column, was also 32 000. E. coli shikimate dehydrogenase is therefore a monomeric NADP-linked dehydrogenase.

Citing Articles

Comparative genomics of seven genomes of genus reveals important halo adaptations and genes for stress response.

Kaur J, Kaur J 3 Biotech. 2024; 14(2):40.

PMID: 38261836 PMC: 10794682. DOI: 10.1007/s13205-023-03887-3.

Regulation of Plant Tannin Synthesis in Crop Species.

Mora J, Pott D, Osorio S, Vallarino J Front Genet. 2022; 13:870976.

PMID: 35586570 PMC: 9108539. DOI: 10.3389/fgene.2022.870976.

Generation of aroE overexpression mutant of Bacillus megaterium for the production of shikimic acid.

Ghosh S, Banerjee U Microb Cell Fact. 2015; 14:69.

PMID: 25981549 PMC: 4490670. DOI: 10.1186/s12934-015-0251-3.

Proteomic analysis of carbon concentrating chemolithotrophic bacteria Serratia sp. for sequestration of carbon dioxide.

Bharti R, Srivastava S, Thakur I PLoS One. 2014; 9(3):e91300.

PMID: 24619032 PMC: 3949746. DOI: 10.1371/journal.pone.0091300.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of shikimate dehydrogenase from Archaeoglobus fulgidus.

Lee H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011; 67(Pt 12):1556-8.

PMID: 22139165 PMC: 3232138. DOI: 10.1107/S1744309111041194.

References

Pittard J, Wallace B . Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol. 1966; 91(4):1494-508. PMC: 316068. DOI: 10.1128/jb.91.4.1494-1508.1966. View

Miozzari G, Yanofsky C . Gene fusion during the evolution of the tryptophan operon in enterobacteriaceae. Nature. 1979; 277(5696):486-9. DOI: 10.1038/277486a0. View

Berlyn M, GILES N . Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria. J Bacteriol. 1969; 99(1):222-30. PMC: 249991. DOI: 10.1128/jb.99.1.222-230.1969. View

Ely B, Pittard J . Aromatic amino acid biosynthesis: regulation of shikimate kinase in Escherichia coli K-12. J Bacteriol. 1979; 138(3):933-43. PMC: 218124. DOI: 10.1128/jb.138.3.933-943.1979. View

ZALKIN H, Yanofsky C . Yeast gene TRP5: structure, function, regulation. J Biol Chem. 1982; 257(3):1491-500. View

Volz K, Matthews D, Alden R, FREER S, HANSCH C, KAUFMAN B . Crystal structure of avian dihydrofolate reductase containing phenyltriazine and NADPH. J Biol Chem. 1982; 257(5):2528-36. View

Hawkins A, Reinert W, GILES N . Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli. Biochem J. 1982; 203(3):769-73. PMC: 1158294. DOI: 10.1042/bj2030769. View

Eschenbruch M, Burk R . Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels. Anal Biochem. 1982; 125(1):96-9. DOI: 10.1016/0003-2697(82)90387-6. View

Lewendon A, Coggins J . Purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli. Biochem J. 1983; 213(1):187-91. PMC: 1152107. DOI: 10.1042/bj2130187. View

10.

Smith D, Coggins J . Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa. Biochem J. 1983; 213(2):405-15. PMC: 1152142. DOI: 10.1042/bj2130405. View

11.

Larimer F, Morse C, Beck A, Cole K, Gaertner F . Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae. Mol Cell Biol. 1983; 3(9):1609-14. PMC: 370014. DOI: 10.1128/mcb.3.9.1609-1614.1983. View

12.

Frost J, Bender J, Kadonaga J, Knowles J . Dehydroquinate synthase from Escherichia coli: purification, cloning, and construction of overproducers of the enzyme. Biochemistry. 1984; 23(19):4470-5. DOI: 10.1021/bi00314a036. View

13.

YANIV H, Gilvarg C . Aromatic biosynthesis. XIV. 5-Dehydroshikimic reductase. J Biol Chem. 1955; 213(2):787-95. View

14.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

15.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

16.

Hautala J, Jacobson J, Case M, GILES N . Purification and characterization of catabolic dehydroquinase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa. J Biol Chem. 1975; 250(15):6008-14. View

17.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

18.

Lumsden J, Coggins J . The subunit structure of the arom multienzyme complex of Neurospora crassa. A possible pentafunctional polypeptide chain. Biochem J. 1977; 161(3):599-607. PMC: 1164546. DOI: 10.1042/bj1610599. View

19.

Gaertner F, Cole K . A cluster-gene: evidence for one gene, one polypeptide, five enzymes. Biochem Biophys Res Commun. 1977; 75(2):259-64. DOI: 10.1016/0006-291x(77)91037-3. View

20.

Koshiba T . Purification of two forms of the associated 3-dehydroquinate hydro-lyase and shikimate:NADP+ oxidoreductase in Phaseolus mungo seedlings. Biochim Biophys Acta. 1978; 522(1):10-8. DOI: 10.1016/0005-2744(78)90317-0. View