» Articles » PMID: 2950851

Purification and Characterization of 3-dehydroquinase from Escherichia Coli

Overview
Journal Biochem J
Specialty Biochemistry
Date 1986 Nov 1
PMID 2950851
Citations 20
Authors
Affiliations
Soon will be listed here.
Abstract

A procedure has been developed for the purification of 3-dehydroquinase from Escherichia coli. Homogeneous enzyme with specific activity 163 units/mg of protein was obtained in 19% overall yield. The subunit Mr estimated from polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 29,000. The native Mr, estimated by gel permeation chromatography on Sephacryl S-200 (superfine) and on TSK G3000SW, was in the range 52,000-58,000, indicating that the enzyme is dimeric. The catalytic properties of the enzyme have been determined and shown to be very similar to those of the biosynthetic 3-dehydroquinase component of the arom multifunctional enzyme of Neurospora crassa.

Citing Articles

Regulation of Plant Tannin Synthesis in Crop Species.

Mora J, Pott D, Osorio S, Vallarino J Front Genet. 2022; 13:870976.

PMID: 35586570 PMC: 9108539. DOI: 10.3389/fgene.2022.870976.


Molecular analysis and essentiality of Aro1 shikimate biosynthesis multi-enzyme in .

Stogios P, Liston S, Semper C, Quade B, Michalska K, Evdokimova E Life Sci Alliance. 2022; 5(8).

PMID: 35512834 PMC: 9074039. DOI: 10.26508/lsa.202101358.


Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244.

Nakamura K, Nagaki K, Matsutani M, Adachi O, Kataoka N, Ano Y Appl Microbiol Biotechnol. 2021; 105(14-15):5883-5894.

PMID: 34390353 DOI: 10.1007/s00253-021-11476-8.


Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway.

Liu C, Liu Y, Sun Q, Jiang C, Liu S AMB Express. 2015; 5:7.

PMID: 25852984 PMC: 4314829. DOI: 10.1186/s13568-014-0087-y.


Identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of Enterococcus faecalis.

Cheung V, Xue B, Hernandez-Valladares M, Go M, Tung A, Aguda A PLoS One. 2014; 9(7):e103598.

PMID: 25072253 PMC: 4114755. DOI: 10.1371/journal.pone.0103598.


References
1.
Eschenbruch M, Burk R . Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels. Anal Biochem. 1982; 125(1):96-9. DOI: 10.1016/0003-2697(82)90387-6. View

2.
Kinghorn J, Hawkins A . Cloning and expression in Escherichia coli K-12 of the biosynthetic dehydroquinase function of the arom cluster gene from the eucaryote, Aspergillus nidulans. Mol Gen Genet. 1982; 186(1):145-52. DOI: 10.1007/BF00422927. View

3.
Smith D, Coggins J . Isolation of a bifunctional domain from the pentafunctional arom enzyme complex of Neurospora crassa. Biochem J. 1983; 213(2):405-15. PMC: 1152142. DOI: 10.1042/bj2130405. View

4.
Larimer F, Morse C, Beck A, Cole K, Gaertner F . Isolation of the ARO1 cluster gene of Saccharomyces cerevisiae. Mol Cell Biol. 1983; 3(9):1609-14. PMC: 370014. DOI: 10.1128/mcb.3.9.1609-1614.1983. View

5.
Frost J, Bender J, Kadonaga J, Knowles J . Dehydroquinate synthase from Escherichia coli: purification, cloning, and construction of overproducers of the enzyme. Biochemistry. 1984; 23(19):4470-5. DOI: 10.1021/bi00314a036. View