Bosea Rubneri Sp. Nov. Isolated from Organically Grown Allium Cepa

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2024 Jun 5

PMID 38839619

Authors

Dominic A Stoll

Christina Grimmler

Birgit Hetzer

Alexandra Masoura

Sabine E Kulling

Melanie Huch

Affiliations

Soon will be listed here.

Abstract

Strain ZW T0_25 was isolated from an onion sample (Allium cepa var. Hytech F1) within a storage trial and proofed to be a novel, aerobic, Gram-stain negative, rod-shaped bacterial strain. Analyses of the 16S rRNA gene sequence and of the whole draft genome sequences, i.e., digital DNA-DNA hybridization (dDDH), Average Nucleotide Identity (ANI) and Average Amino Acid Identity (AAI) showed that this strain represents a new species of the genus Bosea. The genome size of strain ZW T0_25 is 6.19 Mbp, and the GC content is 66.9%. As whole cell sugars, rhamnose, ribose and glucose were identified. Ubiquinone Q-10 is the major respiratory quinone with 97.8%. Polar lipids in strain ZW T0_25 are composed of one phosphatidylethanolamine, one phosphatidylglycerol, one aminophospholipid, two aminolipids, one glycolipid and two phospholipids whereas the fatty acid profile predominantly consists of C18:1 w7c (63.3%), C16:1 w7c (19.5%) and C16:0 (7.1%). Phenotypic traits were tested in the wet lab as well as predicted in silico from genome data. Therefore, according to this polyphasic approach, the new name Bosea rubneri sp. nov. with the type strain ZW T0_25 (= DSM 116094 = LMG 33093) is proposed.

Citing Articles

Validation List no. 219. Valid publication of new names and new combinations effectively published outside the IJSEM.

Oren A, Goker M Int J Syst Evol Microbiol. 2024; 74(9).

PMID: 39360699 PMC: 11447935. DOI: 10.1099/ijsem.0.006452.

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