Sp. Nov. a New Species of Slow-growing Bacteria Isolated from Root Nodules of the Relict Species (Pall.) Poir. Originating from Mongolia

Two Gram-stain-negative strains, RCAM04680 and RCAM04685, were isolated from root nodules of the relict legume (Pall.) Poir. originating from the south-western shore of Lake Khuvsgul (Mongolia). The 16S rRNA gene () sequencing data showed that these novel isolates belong to the genus and are phylogenetically closest to the type strains LMG 26379, LMG 28367, LMG 26221 and LMG 26383 (the -similarity levels were 98.7-98.8 %). The gene of strain RCAM04680 showed the highest sequence similarity to the type strain LMG 26383 (95.4 %), while its gene was closest to that of LMG 26379 (94.4 %). The ITS, and sequences of this isolate were most similar to the LMG 28367 (86.8 % for ITS, 90.4 % for the other genes). The most abundant fatty acid was Cω7 (40.8 %). The whole genomes of strains RCAM04680 and RCAM04685 were identical (100 % average nucleotide identity). The highest average nucleotide identity value (82.8 %) was found between the genome of strain RCAM04680 and LMG 28367. The common ABC genes required for legume nodulation were absent in both strains; however, some other symbiotic , , and genes were detected. Based on the genetic study, as well as analyses of the whole-cell fatty acid compositions and phenotypic properties, a new species, sp. nov. (type strain RCAM04680 (=LMG 31125), is proposed.