Design of Microaerobically Inducible MiniR1 Plasmids

Overview

Journal mLife

Date 2024 May 31

PMID 38818336

Authors

Fabiola Islas

Andrea Sabido

Juan-Carlos Sigala

Alvaro R Lara

Affiliations

Soon will be listed here.

Abstract

Plasmid DNA manufacture is an essential step to produce gene therapy agents and next-generation vaccines. However, little attention has been paid toward developing alternative replicons that can be coupled with large-scale production conditions. Our results demonstrate that the miniR1 replicon can be efficiently induced by oxygen limitation when a copy of the regulatory protein RepA under control of a microaerobic promoter is used. The results are potentially attractive for industrial applications.

Citing Articles

Design of microaerobically inducible miniR1 plasmids.

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