Evaluation of Microbial Globin Promoters for Oxygen-limited Processes Using

Overview

Journal J Biol Eng

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2017 Nov 22

PMID 29158775

Citations 3

Authors

Alvaro R Lara

Karim E Jaen

Juan-Carlos Sigala

Lars Regestein

Jochen Buchs

Affiliations

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Abstract

Oxygen-responsive promoters can be useful for synthetic biology applications, however, information on their characteristics is still limited. Here, we characterized a group of heterologous microaerobic globin promoters in . Globin promoters from , , , , and were used to express the FMN-binding fluorescent protein (FbFP), which is a non-oxygen dependent marker. FbFP fluorescence was monitored online in cultures at maximum oxygen transfer capacities (OTR) of 7 and 11 mmol L h. Different FbFP fluorescence intensities were observed and the OTR affected the induction level and specific fluorescence emission rate (the product of the specific fluorescence intensity multiplied by the specific growth rate) of all promoters. The promoter from displayed the highest fluorescence emission yields (the quotient of the fluorescence intensity divided by the scattered light intensity at every time-point) and rate, and together with the promoters from and , the highest induction ratios. These results show the potential of diverse heterologous globin promoters for oxygen-limited processes using .

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