Temperature-inducible Surface Fibrillae Associated with the Virulence Plasmid of Yersinia Enterocolitica and Yersinia Pseudotuberculosis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1985 Feb 1

PMID 3881355

Citations 23

Authors

G Kapperud

E Namork

H J Skarpeid

Affiliations

Soon will be listed here.

Abstract

When cultivated at 37 degrees C in static broth, human clinical isolates of Yersinia enterocolitica (serogroups O:3, O:8, and O:9) and Yersinia pseudotuberculosis (serogroup O:III) produced numerous nonflagellar surface appendages, which appeared as a lawn of fine fibrillae, each having a diameter of 1.5 to 2.0 nm and a length of 50 to 70 nm. Cultivation at 22 degrees C resulted in complete disappearance of the fibrillae. The phenotypic expression of these appendages was correlated with the presence of the 40- to 48-megadalton virulence plasmid and was strongly affected by the growth medium. Evidence is presented which suggests that these plasmid-mediated, temperature-inducible surface fibrillae are responsible for autoagglutination and are related to production of one prominent, Sarkosyl-insoluble polypeptide of ca. 180 kilodaltons in the bacterial outer membrane.

Citing Articles

Yersinia pestis targets neutrophils via complement receptor 3.

Merritt P, Nero T, Bohman L, Felek S, Krukonis E, Marketon M Cell Microbiol. 2014; 17(5):666-87.

PMID: 25359083 PMC: 4402143. DOI: 10.1111/cmi.12391.

Intranasal inoculation of mice with Yersinia pseudotuberculosis causes a lethal lung infection that is dependent on Yersinia outer proteins and PhoP.

Fisher M, Castillo C, Mecsas J Infect Immun. 2006; 75(1):429-42.

PMID: 17074849 PMC: 1828392. DOI: 10.1128/IAI.01287-06.

Identification of a domain in Yersinia virulence factor YadA that is crucial for extracellular matrix-specific cell adhesion and uptake.

Heise T, Dersch P Proc Natl Acad Sci U S A. 2006; 103(9):3375-80.

PMID: 16488979 PMC: 1413876. DOI: 10.1073/pnas.0507749103.

The YadA protein of Yersinia pseudotuberculosis mediates high-efficiency uptake into human cells under environmental conditions in which invasin is repressed.

Eitel J, Dersch P Infect Immun. 2002; 70(9):4880-91.

PMID: 12183532 PMC: 128239. DOI: 10.1128/IAI.70.9.4880-4891.2002.

Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins.

Hoiczyk E, Roggenkamp A, Reichenbecher M, Lupas A, Heesemann J EMBO J. 2000; 19(22):5989-99.

PMID: 11080146 PMC: 305836. DOI: 10.1093/emboj/19.22.5989.

References

Chang M, Doyle M . Identification of specific outer membrane polypeptides associated with virulent Yersinia enterocolitica. Infect Immun. 1984; 43(2):472-6. PMC: 264319. DOI: 10.1128/iai.43.2.472-476.1984. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Brubaker R . Growth of Pasteurella pseudotuberculosis in simulated intracellular and extracellular environments. J Infect Dis. 1967; 117(5):403-17. DOI: 10.1093/infdis/117.5.403. View

Filip C, Fletcher G, Wulff J, Earhart C . Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973; 115(3):717-22. PMC: 246312. DOI: 10.1128/jb.115.3.717-722.1973. View

Meyers J, Sanchez D, ELWELL L, Falkow S . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976; 127(3):1529-37. PMC: 232949. DOI: 10.1128/jb.127.3.1529-1537.1976. View

Laird W, Cavanaugh D . Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980; 11(4):430-2. PMC: 273419. DOI: 10.1128/jcm.11.4.430-432.1980. View

Gemski P, Lazere J, Casey T . Plasmid associated with pathogenicity and calcium dependency of Yersinia enterocolitica. Infect Immun. 1980; 27(2):682-5. PMC: 550818. DOI: 10.1128/iai.27.2.682-685.1980. View

CARTER P, Zahorchak R, Brubaker R . Plague virulence antigens from Yersinia enterocolitica. Infect Immun. 1980; 28(2):638-40. PMC: 550983. DOI: 10.1128/iai.28.2.638-640.1980. View

Gemski P, Lazere J, Casey T, Wohlhieter J . Presence of a virulence-associated plasmid in Yersinia pseudotuberculosis. Infect Immun. 1980; 28(3):1044-7. PMC: 551057. DOI: 10.1128/iai.28.3.1044-1047.1980. View

10.

Portnoy D, Moseley S, Falkow S . Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infect Immun. 1981; 31(2):775-82. PMC: 351377. DOI: 10.1128/iai.31.2.775-782.1981. View

11.

Ferber D, Brubaker R . Plasmids in Yersinia pestis. Infect Immun. 1981; 31(2):839-41. PMC: 351389. DOI: 10.1128/iai.31.2.839-841.1981. View

12.

Maclagan R, Old D . Haemagglutinins and fimbriae in different serotypes and biotypes of Yersinia enterocolitica. J Appl Bacteriol. 1980; 49(2):353-60. DOI: 10.1111/j.1365-2672.1980.tb05135.x. View

13.

Straley S, Brubaker R . Cytoplasmic and membrane proteins of yersiniae cultivated under conditions simulating mammalian intracellular environment. Proc Natl Acad Sci U S A. 1981; 78(2):1224-8. PMC: 319980. DOI: 10.1073/pnas.78.2.1224. View

14.

Portnoy D, Falkow S . Virulence-associated plasmids from Yersinia enterocolitica and Yersinia pestis. J Bacteriol. 1981; 148(3):877-83. PMC: 216287. DOI: 10.1128/jb.148.3.877-883.1981. View

15.

Namork E, Johansen B . Surface activation of carbon film supports for biological electron microscopy. Ultramicroscopy. 1982; 7(4):321-30. DOI: 10.1016/0304-3991(82)90257-1. View

16.

Bolin I, Norlander L, Wolf-Watz H . Temperature-inducible outer membrane protein of Yersinia pseudotuberculosis and Yersinia enterocolitica is associated with the virulence plasmid. Infect Immun. 1982; 37(2):506-12. PMC: 347563. DOI: 10.1128/iai.37.2.506-512.1982. View

17.

Perry R, Brubaker R . Vwa+ phenotype of Yersinia enterocolitica. Infect Immun. 1983; 40(1):166-71. PMC: 264831. DOI: 10.1128/iai.40.1.166-171.1983. View

18.

MARTINEZ R . Plasmid-mediated and temperature-regulated surface properties of Yersinia enterocolitica. Infect Immun. 1983; 41(3):921-30. PMC: 264589. DOI: 10.1128/iai.41.3.921-930.1983. View

19.

Kapperud G, Lassen J . Relationship of virulence-associated autoagglutination to hemagglutinin production in Yersinia enterocolitica and Yersinia enterocolitica-like bacteria. Infect Immun. 1983; 42(1):163-9. PMC: 264538. DOI: 10.1128/iai.42.1.163-169.1983. View

20.

Faris A, Lindahl M, Ljungh A, Old D, Wadstrom T . Autoaggregating Yersinia enterocolitica express surface fimbriae with high surface hydrophobicity. J Appl Bacteriol. 1983; 55(1):97-100. DOI: 10.1111/j.1365-2672.1983.tb02652.x. View