Detection of PYV+ Yersinia Enterocolitica Isolates by P1 Slide Agglutination

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1990 Nov 1

PMID 2254416

Citations 9

Authors

M P Sory

J Tollenaere

C LASZLO

T Biot

G R Cornelis

G Wauters

Affiliations

Soon will be listed here.

Abstract

Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test.

Citing Articles

Case report: A case of brucellosis misdiagnosed as coronavirus disease 2019/influenza in China.

Qin S, Lv D, Duan R, Zheng X, Bukai A, Lu X Front Public Health. 2023; 11:1186800.

PMID: 37724314 PMC: 10505428. DOI: 10.3389/fpubh.2023.1186800.

Immunogenicity of trimeric autotransporter adhesins and their potential as vaccine targets.

Thibau A, Dichter A, Vaca D, Linke D, Goldman A, Kempf V Med Microbiol Immunol. 2019; 209(3):243-263.

PMID: 31788746 PMC: 7247748. DOI: 10.1007/s00430-019-00649-y.

Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA.

Roggenkamp A, Ackermann N, Jacobi C, Truelzsch K, Hoffmann H, Heesemann J J Bacteriol. 2003; 185(13):3735-44.

PMID: 12813066 PMC: 161578. DOI: 10.1128/JB.185.13.3735-3744.2003.

First isolation of virulent Yersinia enterocolitica O8, biotype 1B in Germany.

Schubert S, Bockemuhl J, Brendler U, Heesemann J Eur J Clin Microbiol Infect Dis. 2003; 22(1):66-8.

PMID: 12582749 DOI: 10.1007/s10096-002-0859-1.

Differential secretion of interleukin-8 by human epithelial cell lines upon entry of virulent or nonvirulent Yersinia enterocolitica.

Schulte R, Wattiau P, Hartland E, Robins-Browne R, Cornelis G Infect Immun. 1996; 64(6):2106-13.

PMID: 8675314 PMC: 174043. DOI: 10.1128/iai.64.6.2106-2113.1996.

References

Laroche Y, Van Bouchaute M, Cornelis G . A restriction map of virulence plasmid pVYE439-80 from a serogroup 9 Yersinia enterocolitica strain. Plasmid. 1984; 12(1):67-70. DOI: 10.1016/0147-619x(84)90069-6. View

Michiels T, Wattiau P, Brasseur R, Ruysschaert J, Cornelis G . Secretion of Yop proteins by Yersiniae. Infect Immun. 1990; 58(9):2840-9. PMC: 313576. DOI: 10.1128/iai.58.9.2840-2849.1990. View

Kapperud G, Namork E, Skarpeid H . Temperature-inducible surface fibrillae associated with the virulence plasmid of Yersinia enterocolitica and Yersinia pseudotuberculosis. Infect Immun. 1985; 47(2):561-6. PMC: 263209. DOI: 10.1128/iai.47.2.561-566.1985. View

Balligand G, Laroche Y, Cornelis G . Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination. Infect Immun. 1985; 48(3):782-6. PMC: 261262. DOI: 10.1128/iai.48.3.782-786.1985. View

Zaleska M, Lounatmaa K, Nurminen M, Wahlstrom E, Makela P . A novel virulence-associated cell surface structure composed of 47-kd protein subunits in Yersinia enterocolitica. EMBO J. 1985; 4(4):1013-8. PMC: 554293. DOI: 10.1002/j.1460-2075.1985.tb03732.x. View

Prpic J, Robins-Browne R, Davey R . In vitro assessment of virulence in Yersinia enterocolitica and related species. J Clin Microbiol. 1985; 22(1):105-10. PMC: 268331. DOI: 10.1128/jcm.22.1.105-110.1985. View

Pulkkinen L, Granberg I, Granfors K, Toivanen A . Restriction map of virulence plasmid in Yersinia enterocolitica O:3. Plasmid. 1986; 16(3):225-7. DOI: 10.1016/0147-619x(86)90062-4. View

Kapperud G, Namork E, Skurnik M, Nesbakken T . Plasmid-mediated surface fibrillae of Yersinia pseudotuberculosis and Yersinia enterocolitica: relationship to the outer membrane protein YOP1 and possible importance for pathogenesis. Infect Immun. 1987; 55(9):2247-54. PMC: 260686. DOI: 10.1128/iai.55.9.2247-2254.1987. View

Nesbakken T, Kapperud G, Sorum H, Dommarsnes K . Structural variability of 40-50 Mdal virulence plasmids from Yersinia enterocolitica. Geographical and ecological distribution of plasmid variants. Acta Pathol Microbiol Immunol Scand B. 1987; 95(3):167-73. DOI: 10.1111/j.1699-0463.1987.tb03107.x. View

10.

Wauters G, Kandolo K, Janssens M . Revised biogrouping scheme of Yersinia enterocolitica. Contrib Microbiol Immunol. 1987; 9:14-21. View

11.

Bolin I, Forsberg A, Norlander L, Skurnik M, Wolf-Watz H . Identification and mapping of the temperature-inducible, plasmid-encoded proteins of Yersinia spp. Infect Immun. 1988; 56(2):343-8. PMC: 259286. DOI: 10.1128/iai.56.2.343-348.1988. View

12.

Sory M, Cornelis G . Yersinia enterocolitica O:9 as a potential live oral carrier for protective antigens. Microb Pathog. 1988; 4(6):431-42. DOI: 10.1016/0882-4010(88)90028-9. View

13.

Cornelis G, Sluiters C, de Rouvroit C, Michiels T . Homology between virF, the transcriptional activator of the Yersinia virulence regulon, and AraC, the Escherichia coli arabinose operon regulator. J Bacteriol. 1989; 171(1):254-62. PMC: 209580. DOI: 10.1128/jb.171.1.254-262.1989. View

14.

Biot T, Cornelis G . The replication, partition and yop regulation of the pYV plasmids are highly conserved in Yersinia enterocolitica and Y. pseudotuberculosis. J Gen Microbiol. 1988; 134(6):1525-34. DOI: 10.1099/00221287-134-6-1525. View

15.

Cornelis G, Vanootegem J, Sluiters C . Transcription of the yop regulon from Y. enterocolitica requires trans acting pYV and chromosomal genes. Microb Pathog. 1987; 2(5):367-79. DOI: 10.1016/0882-4010(87)90078-7. View

16.

Cornelis G, Sory M, Laroche Y, Derclaye I . Genetic analysis of the plasmid region controlling virulence in Yersinia enterocolitica 0:9 by Mini-Mu insertions and lac gene fusions. Microb Pathog. 1986; 1(4):349-59. DOI: 10.1016/0882-4010(86)90067-7. View

17.

Kaneko S, Maruyama T . Evaluation of enzyme immunoassay for the detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis strains. J Clin Microbiol. 1989; 27(4):748-51. PMC: 267410. DOI: 10.1128/jcm.27.4.748-751.1989. View

18.

MARTINEZ R . Thermoregulation-dependent expression of Yersinia enterocolitica protein 1 imparts serum resistance to Escherichia coli K-12. J Bacteriol. 1989; 171(7):3732-9. PMC: 210118. DOI: 10.1128/jb.171.7.3732-3739.1989. View

19.

Cornelis G, Colson C . Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli. J Gen Microbiol. 1975; 87(2):285-91. DOI: 10.1099/00221287-87-2-285. View

20.

Laird W, Cavanaugh D . Correlation of autoagglutination and virulence of yersiniae. J Clin Microbiol. 1980; 11(4):430-2. PMC: 273419. DOI: 10.1128/jcm.11.4.430-432.1980. View