Surface Activation of Carbon Film Supports for Biological Electron Microscopy

Overview

Journal Ultramicroscopy

Publisher Elsevier

Specialty Radiology

Date 1982 Jan 1

PMID 6180533

Citations 5

Authors

E Namork

B V Johansen

Affiliations

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Abstract

The effect of glow-discharging on naked carbon-filmed grids has been evaluated. The effect of different locations of the grids within a DC discharge, operated in the normal glow, was analyzed by applying various biological specimens to the grids. Two locations were found to give consistent results: (a) in Crookes dark space, particulate specimens, negatively stained, spread evenly--suggesting a new negative surface charge of the support; (b) below the anode, nucleic acids, selectively (positively) stained, appeared as well spread filaments, indicating a net positive surface charge.

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