Fabrication of Immobilized Lipases from on Hierarchical Mesoporous Silica for Enzymatic Enrichment of ω-3 Polyunsaturated Fatty Acids by Selective Hydrolysis

Overview

Journal Food Chem X

Date 2024 May 23

PMID 38779499

Authors

Zhe Dong

Jun Jin

Wei Wei

Xiaosan Wang

Gangcheng Wu

Xingguo Wang

Qingzhe Jin

Affiliations

Soon will be listed here.

Abstract

In this study, lipase from immobilized on hydrophobic hierarchical porous hollow silica microsphere (HPHSM-C3) via adsorption. The prepared biocatalyst HPHSM-C3@CRL exhibited higher activity, thermal and pH stability. HPHSM-C3@CRL remained 70.2% of initial activity after 30 days of storage at 24 °C and 50.4% of initial activity after 10 cycles. Moreover, HPHSM-C3@CRL was utilized in enzymatic enrichment of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) in glycerides, achieving ω-3 PUFAs content of 53.42% with the hydrolysis rate of 48.78% under optimal condition. The K and V value of HPHSM-C3@CRL was 42.2% lower and 63.5% higher than those of CRL, respectively. The 3D structure analysis of CRL, substrates and pore structure of HPHSM-C3 suggested that the hierarchical pore improved activity and selectivity of immobilized lipase. This result demonstrated that HPHSM-C3@CRL may be an effective biocatalyst for the enzymatic enrichment of ω-3 PUFAs in food industries.

Citing Articles

A Comprehensive Study for Determination of Free Fatty Acids in Selected Biological Materials: A Review.

Ucar B, Gholami Z, Svobodova K, Hradecka I, Honig V Foods. 2024; 13(12).

PMID: 38928832 PMC: 11203194. DOI: 10.3390/foods13121891.

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