Defective Contributes to Increased Membrane Fluidity and Cell Wall Thickening in with High-level Daptomycin Resistance

Overview

Journal mSphere

Publisher American Society for Microbiology

Specialties Microbiology
Parasitology

Date 2024 May 16

PMID 38752757

Authors

Christian D Freeman

Tayte Hansen

Ramona Urbauer

Brian J Wilkinson

Vineet K Singh

Kelly M Hines

Affiliations

Soon will be listed here.

Abstract

Importance: The cationic lipopeptide antimicrobial daptomycin has become an essential tool for combating infections with that display reduced susceptibility to β-lactams or vancomycin. Since daptomycin's activity is based on interaction with the negatively charged membrane of , routes to daptomycin-resistance occur through mutations in the lipid biosynthetic pathway surrounding phosphatidylglycerols and the regulatory systems that control cell envelope homeostasis. Therefore, there are many avenues to achieve daptomycin resistance and several different, and sometimes contradictory, phenotypes of daptomycin-resistant , including both increased and decreased cell wall thickness and membrane fluidity. This study is significant because it demonstrates the unexpected influence of a lipid biosynthesis gene, , on membrane fluidity and cell wall thickness in with high-level daptomycin resistance.

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