Evaluating the Impact of Vitamin D on NF-κB and JAK/STAT Signaling Pathways in

This study delved into the consequences of prolonged administration of vitamin D on innate immune systems, particularly NF-κB and JAK/STAT, in . The outcomes indicated that vitamin D treatment exhibited a notable capacity to improve the survival of adult flies with compromised immune functions, a condition induced by the loss of PGRP-LB, particularly when the flies were exposed to heat-killed . The mutant line that was treated with heat-killed experienced reduced survival. Treatment of heat-killed -treated with vitamin D resulted in improved survival, and this phenotypic feature might be due to the downregulation of gene expression in the NF-κB and JAK/STAT pathways. However, a higher concentration of vitamin D was associated with decreased survival, potentially linked to intricate immunological responses. The research also underscored the influence of vitamin D on the expression of antioxidant genes, and , indicating an augmented resistance to oxidative stress. Further, this study revealed the effect of vitamin D on the reproductive status of the autoinflammatory model, showing an increase in pupae and adult flies with a treatment of 10 mM vitamin D, suggesting the potential benefits of vitamin D on the reproductive profile. Overall, this study provides preliminary insights into the complex interactions between vitamin D, immune pathways, oxidative responses in the cell, and reproduction in .