High-throughput Lipidomic Profiles Sampled with Electroporation-based Biopsy Differentiate Healthy Skin, Cutaneous Squamous Cell Carcinoma, and Basal Cell Carcinoma

Overview

Journal Skin Res Technol

Specialty Biomedical Engineering

Date 2024 May 9

PMID 38721854

Authors

Leetal Louie

Julia Wise

Ariel Berl

Ofir Shir-Az

Vladimir Kravtsov

Zohar Yakhini

Avshalom Shalom

Alexander Golberg

Edward Vitkin

Affiliations

Soon will be listed here.

Abstract

Background: The incidence rates of cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC) skin cancers are rising, while the current diagnostic process is time-consuming. We describe the development of a novel approach to high-throughput sampling of tissue lipids using electroporation-based biopsy, termed e-biopsy. We report on the ability of the e-biopsy technique to harvest large amounts of lipids from human skin samples.

Materials And Methods: Here, 168 lipids were reliably identified from 12 patients providing a total of 13 samples. The extracted lipids were profiled with ultra-performance liquid chromatography and tandem mass spectrometry (UPLC-MS-MS) providing cSCC, BCC, and healthy skin lipidomic profiles.

Results: Comparative analysis identified 27 differentially expressed lipids (p < 0.05). The general profile trend is low diglycerides in both cSCC and BCC, high phospholipids in BCC, and high lyso-phospholipids in cSCC compared to healthy skin tissue samples.

Conclusion: The results contribute to the growing body of knowledge that can potentially lead to novel insights into these skin cancers and demonstrate the potential of the e-biopsy technique for the analysis of lipidomic profiles of human skin tissues.

Citing Articles

High-throughput lipidomic profiles sampled with electroporation-based biopsy differentiate healthy skin, cutaneous squamous cell carcinoma, and basal cell carcinoma.

Louie L, Wise J, Berl A, Shir-Az O, Kravtsov V, Yakhini Z Skin Res Technol. 2024; 30(5):e13706.

PMID: 38721854 PMC: 11079884. DOI: 10.1111/srt.13706.

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