Recent Advances in Analytical Techniques for High Throughput Experimentation

Overview

Journal Anal Sci Adv

Specialty Biotechnology

Date 2024 May 8

PMID 38716456

Authors

Nico Vervoort

Karel Goossens

Mattijs Baeten

Qinghao Chen

Affiliations

Soon will be listed here.

Abstract

High throughput experimentation is a growing and evolving field that allows to execute dozens to several thousands of experiments per day with relatively limited resources. Through miniaturization, typically a high degree of automation and the use of digital data tools, many parallel reactions or experiments at a time can be run in such workflows. High throughput experimentation also requires fast analytical techniques capable of generating critically important analytical data in line with the increased rate of experimentation. As traditional techniques usually do not deliver the speed required, some unique approaches are required to enable workflows to function as designed. This review covers the recent developments (2019-2020) in this field and was intended to give a comprehensive overview of the current "state-of-the-art."

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References

Fialkov A, Lehotay S, Amirav A . Less than one minute low-pressure gas chromatography - mass spectrometry. J Chromatogr A. 2019; 1612:460691. DOI: 10.1016/j.chroma.2019.460691. View

Xiang P, Yang Y, Zhao Z, Chen A, Liu S . Experimentally Validating Open Tubular Liquid Chromatography for a Peak Capacity of 2000 in 3 h. Anal Chem. 2019; 91(16):10518-10523. DOI: 10.1021/acs.analchem.9b01465. View

Morato N, Holden D, Cooks R . High-Throughput Label-Free Enzymatic Assays Using Desorption Electrospray-Ionization Mass Spectrometry. Angew Chem Int Ed Engl. 2020; 59(46):20459-20464. DOI: 10.1002/anie.202009598. View

Holland-Moritz D, Wismer M, Mann B, Farasat I, Devine P, Guetschow E . Mass Activated Droplet Sorting (MADS) Enables High-Throughput Screening of Enzymatic Reactions at Nanoliter Scale. Angew Chem Int Ed Engl. 2019; 59(11):4470-4477. DOI: 10.1002/anie.201913203. View

Fedick P, Iyer K, Wei Z, Avramova L, Capek G, Cooks R . Screening of the Suzuki Cross-Coupling Reaction Using Desorption Electrospray Ionization in High-Throughput and in Leidenfrost Droplet Experiments. J Am Soc Mass Spectrom. 2019; 30(10):2144-2151. DOI: 10.1007/s13361-019-02287-3. View

Wleklinski M, Loren B, Ferreira C, Jaman Z, Avramova L, Sobreira T . High throughput reaction screening using desorption electrospray ionization mass spectrometry. Chem Sci. 2018; 9(6):1647-1653. PMC: 5887808. DOI: 10.1039/c7sc04606e. View

Dores-Sousa J, Terryn H, Eeltink S . Morphology optimization and assessment of the performance limits of high-porosity nanostructured polymer monolithic capillary columns for proteomics analysis. Anal Chim Acta. 2020; 1124:176-183. DOI: 10.1016/j.aca.2020.05.019. View

Belov A, Kozole J, Bean M, Machutta C, Zhang G, Gao E . Acoustic Mist Ionization-Mass Spectrometry: A Comparison to Conventional High-Throughput Screening and Compound Profiling Platforms. Anal Chem. 2020; 92(20):13847-13854. DOI: 10.1021/acs.analchem.0c02508. View

Wouters B, Davydova E, Wouters S, Vivo-Truyols G, Schoenmakers P, Eeltink S . Towards ultra-high peak capacities and peak-production rates using spatial three-dimensional liquid chromatography. Lab Chip. 2015; 15(23):4415-22. DOI: 10.1039/c5lc01169h. View

10.

Sun S, Kennedy R . Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors. Anal Chem. 2014; 86(18):9309-14. PMC: 4165461. DOI: 10.1021/ac502542z. View

11.

Pu F, Elsen N, Williams J . Emerging Chromatography-Free High-Throughput Mass Spectrometry Technologies for Generating Hits and Leads. ACS Med Chem Lett. 2020; 11(11):2108-2113. PMC: 7667647. DOI: 10.1021/acsmedchemlett.0c00314. View

12.

Li Y, Hu Y, Logsdon D, Liu Y, Zhao Y, Cooks R . Accelerated Forced Degradation of Therapeutic Peptides in Levitated Microdroplets. Pharm Res. 2020; 37(7):138. DOI: 10.1007/s11095-020-02868-y. View

13.

Zheng R, Li L, Deng X, Tian M, Wang Z, Yang L . Fully automated chip-based nanoelectrospray ionization-mass spectrometry as an effective tool for rapid and high-throughput screening of 5α-reductase inhibitors. Anal Bioanal Chem. 2020; 412(7):1685-1692. DOI: 10.1007/s00216-020-02408-y. View

14.

Kaplitz A, Kresge G, Selover B, Horvat L, Franklin E, Godinho J . High-Throughput and Ultrafast Liquid Chromatography. Anal Chem. 2019; 92(1):67-84. DOI: 10.1021/acs.analchem.9b04713. View

15.

Herrera B, Moor S, McVeigh M, Roesner E, Marini F, Anslyn E . Rapid Optical Determination of Enantiomeric Excess, Diastereomeric Excess, and Total Concentration Using Dynamic-Covalent Assemblies: A Demonstration Using 2-Aminocyclohexanol and Chemometrics. J Am Chem Soc. 2019; 141(28):11151-11160. PMC: 7497656. DOI: 10.1021/jacs.9b03844. View

16.

Komendova M, Nawada S, Metelka R, Schoenmakers P, Urban J . Multichannel separation device with parallel electrochemical detection. J Chromatogr A. 2019; 1610:460537. DOI: 10.1016/j.chroma.2019.460537. View

17.

Habe T, Liu C, Covey T, Simon R, Reindl W, Buttner F . Ultrahigh-Throughput ESI-MS: Sampling Pushed to Six Samples per Second by Acoustic Ejection Mass Spectrometry. Anal Chem. 2020; 92(18):12242-12249. DOI: 10.1021/acs.analchem.0c01632. View

18.

Krenkel H, Hartmane E, Piras C, Brown J, Morris M, Cramer R . Advancing Liquid Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Toward Ultrahigh-Throughput Analysis. Anal Chem. 2020; 92(4):2931-2936. PMC: 7145281. DOI: 10.1021/acs.analchem.9b05202. View

19.

Herrera B, Pilicer S, Anslyn E, Joyce L, Wolf C . Optical Analysis of Reaction Yield and Enantiomeric Excess: A New Paradigm Ready for Prime Time. J Am Chem Soc. 2018; 140(33):10385-10401. DOI: 10.1021/jacs.8b06607. View

20.

Boulgakov A, Moor S, Jo H, Metola P, Joyce L, Marcotte E . Next-Generation TLC: A Quantitative Platform for Parallel Spotting and Imaging. J Org Chem. 2020; 85(15):9447-9453. PMC: 7748073. DOI: 10.1021/acs.joc.0c00349. View