Degron Tagging for Rapid Protein Degradation in Mice

Overview

Journal Dis Model Mech

Specialty General Medicine

Date 2024 Apr 26

PMID 38666498

Authors

Brianda A Hernandez-Moran

Gillian Taylor

Alvaro Lorente-Macias

Andrew J Wood

Affiliations

Soon will be listed here.

Abstract

Degron tagging allows proteins of interest to be rapidly degraded, in a reversible and tuneable manner, in response to a chemical stimulus. This provides numerous opportunities for understanding disease mechanisms, modelling therapeutic interventions and constructing synthetic gene networks. In recent years, many laboratories have applied degron tagging successfully in cultured mammalian cells, spurred by rapid advances in the fields of genome editing and targeted protein degradation. In this At a Glance article, we focus on recent efforts to apply degron tagging in mouse models, discussing the distinct set of challenges and opportunities posed by the in vivo environment.

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