Controls Myogenesis by Cis-modulation of in Cattle

Long non-coding RNAs (lncRNAs) have been shown to be involved in the regulation of skeletal muscle development through multiple mechanisms. The present study revealed that the (SRY-box transcription factor 6 antisense upstream) is reverse transcribed from upstream of the bovine sex-determining region Y (SRY)-related high-mobility-group box 6 () gene. was significantly differentially expressed in muscle tissue among different developmental stages in Xianan cattle. Subsequently, knockdown and overexpression experiments discovered that promoted primary skeletal muscle cells proliferation, apoptosis, and differentiation in bovine. The overexpression of in bovine primary skeletal muscle cells resulted in 483 differentially expressed genes (DEGs), including 224 upregulated DEGs and 259 downregulated DEGs. GO functional annotation analysis showed that muscle development-related biological processes such as muscle structure development and muscle cell proliferation were significantly enriched. KEGG pathway analysis revealed that the PI3K/AKT and MAPK signaling pathways were important pathways for DEG enrichment. Notably, we found that inhibited the mRNA and protein expression levels of the gene. Moreover, knockdown of the gene promoted the proliferation and apoptosis of bovine primary skeletal muscle cells. Finally, we showed that promoted the proliferation and apoptosis of bovine primary skeletal muscle cells by cis-modulation of in cattle. This work illustrates our discovery of the molecular mechanisms underlying the regulation of in the development of beef.