USF1-induced Upregulation of LINC01048 Promotes Cell Proliferation and Apoptosis in Cutaneous Squamous Cell Carcinoma by Binding to TAF15 to Transcriptionally Activate YAP1

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2019 Apr 2

PMID 30931936

Citations 28

Authors

Lezi Chen

Quan Chen

Shifeng Kuang

Chengli Zhao

Lu Yang

Yi Zhang

Huilan Zhu

Ridong Yang

Affiliations

Soon will be listed here.

Abstract

Previous studies have revealed that dysregulation of long non-coding RNAs (lncRNAs) can facilitate carcinogenesis. This study aims to investigate the biological role of a certain lncRNA in cutaneous squamous cell carcinoma (CSCC). According to the data of TCGA database, high expression of long intergenic non-protein coding RNA 1048 (LINC01048) is an unfavorable prognostic factor for patients with CSCC. Therefore, we further detected the expression pattern of LINC01048 in CSCC tissues. Obviously, LINC01048 was expressed higher in the CSCC tissues and recurrence tissues compared with that in adjacent normal tissues and non-recurrence tissues. Furthermore, Kaplan-Meier analysis revealed the negative correlation between LINC01048 expression and the overall survival and disease-free survival of CSCC patients. Subsequently, functional assays were conducted to prove the inhibitory effect of silenced LINC01048 on the proliferation and apoptosis of CSCC cells. Mechanistically, LINC01048 was proved to be transcriptionally activated by USF1. Pathway analysis and western blot assay showed that knockdown of LINC01048 led to the activation of Hippo pathway. Moreover, YAP1, a Hippo pathway factor, was positively regulated by LINC01048. Further mechanism investigation revealed that LINC01048 increased the binding of TAF15 to YAP1 promoter to transcriptionally activate YAP1 in CSCC cells. Finally, rescue assays demonstrated that YAP1 involved in LINC01048-mediated CSCC cell proliferation and apoptosis. In conclusion, USF1-induced upregulation of LINC01048 promoted CSCC by interacting with TAF15 to upregulate YAP1.

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References

Wang K, Chang H . Molecular mechanisms of long noncoding RNAs. Mol Cell. 2011; 43(6):904-14. PMC: 3199020. DOI: 10.1016/j.molcel.2011.08.018. View

Fatica A, Bozzoni I . Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet. 2013; 15(1):7-21. DOI: 10.1038/nrg3606. View

Krchnakova Z, Thakur P, Krausova M, Bieberstein N, Haberman N, Muller-McNicoll M . Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5' splice-site sequences due to weak interactions with SR proteins. Nucleic Acids Res. 2018; 47(2):911-928. PMC: 6344860. DOI: 10.1093/nar/gky1147. View

Johnson R, Halder G . The two faces of Hippo: targeting the Hippo pathway for regenerative medicine and cancer treatment. Nat Rev Drug Discov. 2013; 13(1):63-79. PMC: 4167640. DOI: 10.1038/nrd4161. View

Piipponen M, Nissinen L, Farshchian M, Riihila P, Kivisaari A, Kallajoki M . Long Noncoding RNA PICSAR Promotes Growth of Cutaneous Squamous Cell Carcinoma by Regulating ERK1/2 Activity. J Invest Dermatol. 2016; 136(8):1701-1710. DOI: 10.1016/j.jid.2016.03.028. View

Zhou L, Wang Y, Ou C, Lin Z, Wang J, Liu H . microRNA-365-targeted nuclear factor I/B transcriptionally represses cyclin-dependent kinase 6 and 4 to inhibit the progression of cutaneous squamous cell carcinoma. Int J Biochem Cell Biol. 2015; 65:182-91. DOI: 10.1016/j.biocel.2015.06.009. View

Zhou J, Liu R, Luo C, Zhou X, Xia K, Chen X . MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1. Cancer Biol Ther. 2014; 15(10):1340-9. PMC: 4130727. DOI: 10.4161/cbt.29821. View

Chen Q, Chen X, Chen Z, Nie F, Wei C, Ma H . Long intergenic non-coding RNA 00152 promotes lung adenocarcinoma proliferation via interacting with EZH2 and repressing IL24 expression. Mol Cancer. 2017; 16(1):17. PMC: 5251237. DOI: 10.1186/s12943-017-0581-3. View

Zhang M, Zhao Y, Zhang Y, Wang D, Gu S, Feng W . LncRNA UCA1 promotes migration and invasion in pancreatic cancer cells via the Hippo pathway. Biochim Biophys Acta Mol Basis Dis. 2018; 1864(5 Pt A):1770-1782. DOI: 10.1016/j.bbadis.2018.03.005. View

10.

Zhou Y, Shan T, Ding W, Hua Z, Shen Y, Lu Z . Study on mechanism about long noncoding RNA MALAT1 affecting pancreatic cancer by regulating Hippo-YAP signaling. J Cell Physiol. 2017; 233(8):5805-5814. DOI: 10.1002/jcp.26357. View

11.

Kung J, Colognori D, Lee J . Long noncoding RNAs: past, present, and future. Genetics. 2013; 193(3):651-69. PMC: 3583990. DOI: 10.1534/genetics.112.146704. View

12.

Liu X, Li D, Zhang W, Guo M, Zhan Q . Long non-coding RNA gadd7 interacts with TDP-43 and regulates Cdk6 mRNA decay. EMBO J. 2012; 31(23):4415-27. PMC: 3512391. DOI: 10.1038/emboj.2012.292. View

13.

Singh A, Willems E, Singh A, Hafeez B, Ong I, Mehta S . Ultraviolet radiation-induced tumor necrosis factor alpha, which is linked to the development of cutaneous SCC, modulates differential epidermal microRNAs expression. Oncotarget. 2016; 7(14):17945-56. PMC: 4951262. DOI: 10.18632/oncotarget.7595. View

14.

Wapinski O, Chang H . Long noncoding RNAs and human disease. Trends Cell Biol. 2011; 21(6):354-61. DOI: 10.1016/j.tcb.2011.04.001. View

15.

Hu G, Dong B, Zhang J, Zhai W, Xie T, Huang B . The long noncoding RNA HOTAIR activates the Hippo pathway by directly binding to SAV1 in renal cell carcinoma. Oncotarget. 2017; 8(35):58654-58667. PMC: 5601682. DOI: 10.18632/oncotarget.17414. View

16.

Li S, Zhou L, Gao L, Wang Y, Ding Z . [Role of long noncoding RNA MALAT1 promotes the occurrence and progression of cutaneous squamous cell carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao. 2018; 38(4):421-427. PMC: 6765650. View

17.

Deng G, Sui G . Noncoding RNA in oncogenesis: a new era of identifying key players. Int J Mol Sci. 2013; 14(9):18319-49. PMC: 3794782. DOI: 10.3390/ijms140918319. View

18.

Zhang L, Xiang P, Han X, Wu L, Li X, Xiong Z . Decreased expression of microRNA-20a promotes tumor progression and predicts poor prognosis of cutaneous squamous cell carcinoma. Int J Clin Exp Pathol. 2015; 8(9):11446-51. PMC: 4637689. View

19.

Wei S, Zhao M, Wang X, Li Y, Wang K . PU.1 controls the expression of long noncoding RNA HOTAIRM1 during granulocytic differentiation. J Hematol Oncol. 2016; 9(1):44. PMC: 4857283. DOI: 10.1186/s13045-016-0274-1. View

20.

Leichter M, Marko M, Ganou V, Patrinou-Georgoula M, Tora L, Guialis A . A fraction of the transcription factor TAF15 participates in interactions with a subset of the spliceosomal U1 snRNP complex. Biochim Biophys Acta. 2011; 1814(12):1812-24. DOI: 10.1016/j.bbapap.2011.09.008. View