Exploring GPCR Conformational Dynamics Using Single-molecule Fluorescence

Overview

Journal Methods

Specialty Biochemistry

Date 2024 Apr 11

PMID 38604413

Authors

Eugene Agyemang

Alyssa N Gonneville

Sriram Tiruvadi-Krishnan

Rajan Lamichhane

Affiliations

Soon will be listed here.

Abstract

G protein-coupled receptors (GPCRs) are membrane proteins that transmit specific external stimuli into cells by changing their conformation. This conformational change allows them to couple and activate G-proteins to initiate signal transduction. A critical challenge in studying and inferring these structural dynamics arises from the complexity of the cellular environment, including the presence of various endogenous factors. Due to the recent advances in cell-expression systems, membrane-protein purification techniques, and labeling approaches, it is now possible to study the structural dynamics of GPCRs at a single-molecule level both in vitro and in live cells. In this review, we discuss state-of-the-art techniques and strategies for expressing, purifying, and labeling GPCRs in the context of single-molecule research. We also highlight four recent studies that demonstrate the applications of single-molecule microscopy in revealing the dynamics of GPCRs. These techniques are also useful as complementary methods to verify the results obtained from other structural biology tools like cryo-electron microscopy and x-ray crystallography.

Citing Articles

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