Bulk RNA Sequencing for Analysis of Post COVID-19 Condition in Adolescents and Young Adults

Overview

Journal J Transl Med

Publisher Biomed Central

Specialties Biomedical Engineering
General Medicine

Date 2024 Mar 27

PMID 38532465

Authors

Silke Lauren Sommen

Zhi Zhao

Sunniva Segtnan

Tonje Stiansen-Sonerud

Joel Selvakumar

Lise Beier Havdal

Johannes Gjerstad

Vegard Bruun Bratholm Wyller

Lise Lund Berven

Affiliations

Soon will be listed here.

Abstract

Background: Post COVID-19 condition (PCC) is a complication of SARS-COV-2 infection and can lead to long-term disability.

Methods: The present study was designed to analyse the gene expression patterns of PCC through bulk RNA sequencing of whole blood and to explore the potential molecular mechanisms of PCC. Whole blood was collected from 80 participants enrolled in a prospective cohort study following SARS-CoV-2 infected and non-infected individuals for 6 months after recruitment and was used for bulk RNA sequencing. Identification of differentially expressed genes (DEG), pathway enrichment and immune cell deconvolution was performed to explore potential biological pathways involved in PCC.

Results: We have found 13 differentially expressed genes associated with PCC. Enriched pathways were related to interferon-signalling and anti-viral immune processes.

Conclusion: The PCC transcriptome is characterized by a modest overexpression of interferon-stimulated genes, pointing to a subtle ongoing inflammatory response.

Citing Articles

Unlocking the Potential of RNA Sequencing in COVID-19: Toward Accurate Diagnosis and Personalized Medicine.

Eldien H, Almaeen A, El Fath A, Taha A, Ahmed R, Elfadil H Diagnostics (Basel). 2025; 15(2).

PMID: 39857114 PMC: 11763845. DOI: 10.3390/diagnostics15020229.

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