Multimodal Control of Bacterial Gene Expression by Red and Blue Light

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Mar 12

PMID 38468104

Authors

Stefanie S M Meier

Elina Multamaki

Americo T Ranzani

Heikki Takala

Andreas Moglich

Affiliations

Soon will be listed here.

Abstract

By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.

Citing Articles

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Meier S, Multamaki E, Ranzani A, Takala H, Moglich A Nat Commun. 2024; 15(1):4876.

PMID: 38858359 PMC: 11164954. DOI: 10.1038/s41467-024-49251-8.

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