Noninvasive Visualization of Amyloid-Beta Deposits in Alzheimer's Amyloidosis Mice Via Fluorescence Molecular Tomography Using Contrast Agent

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2024 Mar 1

PMID 38427199

Authors

Wuwei Ren

Ruiqing Ni

Affiliations

Soon will be listed here.

Abstract

Alzheimer's disease is pathologically featured by the accumulation of amyloid-beta (Aβ) plaque and neurofibrillary tangles. Compared to small animal positron emission tomography, optical imaging features nonionizing radiation, low cost, and logistic convenience. Optical detection of Aβ deposits is typically implemented by 2D macroscopic imaging and various microscopic techniques assisted with Aβ-targeted contrast agents. Here, we introduce fluorescence molecular tomography (FMT), a macroscopic 3D fluorescence imaging technique, convenient for in vivo longitudinal monitoring of the animal brain without the involvement of cranial window opening operation. This chapter aims to provide the protocols for FMT in vivo imaging of Aβ deposits in the brain of rodent model of Alzheimer's disease. The materials, stepwise method, notes, limitations of FMT, and emerging opportunities for FMT techniques are presented.

References

Perani D, Iaccarino L, Jacobs A . Application of advanced brain positron emission tomography-based molecular imaging for a biological framework in neurodegenerative proteinopathies. Alzheimers Dement (Amst). 2019; 11:327-332. PMC: 6505113. DOI: 10.1016/j.dadm.2019.02.004. View

Noltes M, van Dam G, Nagengast W, van der Zaag P, Slart R, Szymanski W . Let's embrace optical imaging: a growing branch on the clinical molecular imaging tree. Eur J Nucl Med Mol Imaging. 2021; 48(13):4120-4128. DOI: 10.1007/s00259-021-05476-z. View

Rodriguez-Vieitez E, Ni R, Gulyas B, Toth M, Haggkvist J, Halldin C . Astrocytosis precedes amyloid plaque deposition in Alzheimer APPswe transgenic mouse brain: a correlative positron emission tomography and in vitro imaging study. Eur J Nucl Med Mol Imaging. 2015; 42(7):1119-32. PMC: 4424277. DOI: 10.1007/s00259-015-3047-0. View

Yan C, Dai J, Yao Y, Fu W, Tian H, Zhu W . Preparation of near-infrared AIEgen-active fluorescent probes for mapping amyloid-β plaques in brain tissues and living mice. Nat Protoc. 2023; 18(4):1316-1336. DOI: 10.1038/s41596-022-00789-1. View

Nguyen D, Uhlmann V, Planchette A, Marchand P, Van De Ville D, Lasser T . Supervised learning to quantify amyloidosis in whole brains of an Alzheimer's disease mouse model acquired with optical projection tomography. Biomed Opt Express. 2019; 10(6):3041-3060. PMC: 6583328. DOI: 10.1364/BOE.10.003041. View

Calvo-Rodriguez M, Hou S, Snyder A, Dujardin S, Shirani H, Nilsson K . In vivo detection of tau fibrils and amyloid β aggregates with luminescent conjugated oligothiophenes and multiphoton microscopy. Acta Neuropathol Commun. 2019; 7(1):171. PMC: 6839235. DOI: 10.1186/s40478-019-0832-1. View

Ni R, Chen Z, Gerez J, Shi G, Zhou Q, Riek R . Detection of cerebral tauopathy in P301L mice using high-resolution large-field multifocal illumination fluorescence microscopy. Biomed Opt Express. 2020; 11(9):4989-5002. PMC: 7510859. DOI: 10.1364/BOE.395803. View

Wang L, Yao J . A practical guide to photoacoustic tomography in the life sciences. Nat Methods. 2016; 13(8):627-38. PMC: 4980387. DOI: 10.1038/nmeth.3925. View

Hou S, Yang J, Lee J, Kwon Y, Calvo-Rodriguez M, Bao K . Near-infrared fluorescence lifetime imaging of amyloid-β aggregates and tau fibrils through the intact skull of mice. Nat Biomed Eng. 2023; 7(3):270-280. PMC: 10040441. DOI: 10.1038/s41551-023-01003-7. View

10.

Ntziachristos V, Tung C, Bremer C, Weissleder R . Fluorescence molecular tomography resolves protease activity in vivo. Nat Med. 2002; 8(7):757-60. DOI: 10.1038/nm729. View

11.

Ren W, Isler H, Wolf M, Ripoll J, Rudin M . Smart Toolkit for Fluorescence Tomography: Simulation, Reconstruction, and Validation. IEEE Trans Biomed Eng. 2019; 67(1):16-26. DOI: 10.1109/TBME.2019.2907460. View

12.

Stuker F, Ripoll J, Rudin M . Fluorescence molecular tomography: principles and potential for pharmaceutical research. Pharmaceutics. 2013; 3(2):229-74. PMC: 3864234. DOI: 10.3390/pharmaceutics3020229. View

13.

Arridge S, Dehghani H, Schweiger M, Okada E . The finite element model for the propagation of light in scattering media: a direct method for domains with nonscattering regions. Med Phys. 2000; 27(1):252-64. DOI: 10.1118/1.598868. View

14.

Liu X, He X, Yan Z, Lu H . 4-D reconstruction of fluorescence molecular tomography using re-assembled measurement data. Biomed Opt Express. 2015; 6(6):1963-76. PMC: 4473737. DOI: 10.1364/BOE.6.001963. View

15.

Hyde D, de Kleine R, MacLaurin S, Miller E, Brooks D, Krucker T . Hybrid FMT-CT imaging of amyloid-beta plaques in a murine Alzheimer's disease model. Neuroimage. 2008; 44(4):1304-11. DOI: 10.1016/j.neuroimage.2008.10.038. View

16.

Ren W, Li L, Zhang J, Vaas M, Klohs J, Ripoll J . Non-invasive visualization of amyloid-beta deposits in Alzheimer amyloidosis mice using magnetic resonance imaging and fluorescence molecular tomography. Biomed Opt Express. 2022; 13(7):3809-3822. PMC: 9352276. DOI: 10.1364/BOE.458290. View

17.

Peng C, Wang X, Li Y, Li H, Wong M . Versatile fluorescent probes for near-infrared imaging of amyloid-β species in Alzheimer's disease mouse model. J Mater Chem B. 2020; 7(12):1986-1995. DOI: 10.1039/c9tb00161a. View

18.

Darne C, Lu Y, Sevick-Muraca E . Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update. Phys Med Biol. 2013; 59(1):R1-64. DOI: 10.1088/0031-9155/59/1/R1. View

19.

Ren W, Ji B, Guan Y, Cao L, Ni R . Recent Technical Advances in Accelerating the Clinical Translation of Small Animal Brain Imaging: Hybrid Imaging, Deep Learning, and Transcriptomics. Front Med (Lausanne). 2022; 9:771982. PMC: 8987112. DOI: 10.3389/fmed.2022.771982. View

20.

Ren W, Jiang J, Di Costanzo Mata A, Kalyanov A, Ripoll J, Lindner S . Multimodal imaging combining time-domain near-infrared optical tomography and continuous-wave fluorescence molecular tomography. Opt Express. 2020; 28(7):9860-9874. DOI: 10.1364/OE.385392. View