Simultaneous Detection of Exosomal MicroRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2024 Feb 24

PMID 38398977

Authors

Su Bin Han

Soo Suk Lee

Affiliations

Soon will be listed here.

Abstract

We present a surface acoustic wave (SAW) sensor array for microRNA (miRNA) detection that utilizes photocatalytic silver staining on titanium dioxide (TiO) nanoparticles as a signal enhancement technique for high sensitivity with an internal reference sensor for high reproducibility. A sandwich hybridization was performed on working sensors of the SAW sensor array that could simultaneously capture and detect three miRNAs (miRNA-21, miRNA-106b, and miRNA-155) known to be upregulated in cancer. Sensor responses due to signal amplification varied depending on the concentration of synthetic miRNAs. It was confirmed that normalization (a ratio of working sensor response to reference sensor response) screened out background interferences by manipulating data and minimized non-uniformity in the photocatalytic silver staining step by suppressing disturbances to both working sensor signal and reference sensor signal. Finally, we were able to successfully detect target miRNAs in cancer cell-derived exosomal miRNAs with performance comparable to the detection of synthetic miRNAs.

Citing Articles

A Versatile SAW Sensor-Based Modular and Portable Platform for a Multi-Sensor Device.

Lopez-Luna A, Arroyo P, Matatagui D, Sanchez-Vicente C, Lozano J Micromachines (Basel). 2025; 16(2).

PMID: 40047631 PMC: 11857818. DOI: 10.3390/mi16020170.

Recent Advances in SAW Sensors for Detection of Cancer Biomarkers.

Aleixandre M, Horrillo M Biosensors (Basel). 2025; 15(2).

PMID: 39996991 PMC: 11852368. DOI: 10.3390/bios15020088.

Isolation and Characterization of Exosomes from Cancer Cells Using Antibody-Functionalized Paddle Screw-Type Devices and Detection of Exosomal miRNA Using Piezoelectric Biosensor.

Han S, Lee S Sensors (Basel). 2024; 24(16).

PMID: 39205093 PMC: 11359151. DOI: 10.3390/s24165399.

References

Kaur J, Singh K, Boro R, Thampi K, Raje M, Varshney G . Immunochromatographic dipstick assay format using gold nanoparticles labeled protein-hapten conjugate for the detection of atrazine. Environ Sci Technol. 2007; 41(14):5028-36. DOI: 10.1021/es070194j. View

Osborne C, Hobbs K, Trent J . Biological differences among MCF-7 human breast cancer cell lines from different laboratories. Breast Cancer Res Treat. 1987; 9(2):111-21. DOI: 10.1007/BF01807363. View

Zouari M, Campuzano S, Pingarron J, Raouafi N . Amperometric Biosensing of miRNA-21 in Serum and Cancer Cells at Nanostructured Platforms Using Anti-DNA-RNA Hybrid Antibodies. ACS Omega. 2019; 3(8):8923-8931. PMC: 6644860. DOI: 10.1021/acsomega.8b00986. View

Premaratne G, Al Mubarak Z, Senavirathna L, Liu L, Krishnan S . Measuring Ultra-low Levels of Nucleotide Biomarkers Using Quartz Crystal Microbalance and SPR Microarray Imaging Methods: A Comparative Analysis. Sens Actuators B Chem. 2017; 253:368-375. PMC: 5703433. DOI: 10.1016/j.snb.2017.06.138. View

Volinia S, Calin G, Liu C, Ambs S, Cimmino A, Petrocca F . A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A. 2006; 103(7):2257-61. PMC: 1413718. DOI: 10.1073/pnas.0510565103. View

Palaniappan A, Cheema J, Rajwar D, Ammanath G, Xiaohu L, Koon L . Polythiophene derivative on quartz resonators for miRNA capture and assay. Analyst. 2015; 140(23):7912-7. DOI: 10.1039/c5an01663k. View

Kim D, Daniel W, Mirkin C . Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes. Anal Chem. 2009; 81(21):9183-7. PMC: 3200545. DOI: 10.1021/ac9018389. View

Li N, Miao Y, Shan Y, Liu B, Li Y, Zhao L . MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer. Cell Death Dis. 2017; 8(5):e2796. PMC: 5520687. DOI: 10.1038/cddis.2017.119. View

Lange K, Rapp B, Rapp M . Surface acoustic wave biosensors: a review. Anal Bioanal Chem. 2008; 391(5):1509-19. DOI: 10.1007/s00216-008-1911-5. View

10.

Rabinowits G, Gercel-Taylor C, Day J, Taylor D, Kloecker G . Exosomal microRNA: a diagnostic marker for lung cancer. Clin Lung Cancer. 2009; 10(1):42-6. DOI: 10.3816/CLC.2009.n.006. View

11.

Li X, Carthew R . A microRNA mediates EGF receptor signaling and promotes photoreceptor differentiation in the Drosophila eye. Cell. 2005; 123(7):1267-77. DOI: 10.1016/j.cell.2005.10.040. View

12.

Ciesla M, Skrzypek K, Kozakowska M, Loboda A, Jozkowicz A, Dulak J . MicroRNAs as biomarkers of disease onset. Anal Bioanal Chem. 2011; 401(7):2051-61. DOI: 10.1007/s00216-011-5001-8. View

13.

Gizeli E, Stevenson A, Goddard N, Lowe C . A novel Love-plate acoustic sensor utilizing polymer overlayers. IEEE Trans Ultrason Ferroelectr Freq Control. 1992; 39(5):657-9. DOI: 10.1109/58.156185. View

14.

Brennecke J, Hipfner D, Stark A, Russell R, Cohen S . bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell. 2003; 113(1):25-36. DOI: 10.1016/s0092-8674(03)00231-9. View

15.

Hong M, Sun H, Xu L, Yue Q, Shen G, Li M . In situ monitoring of cytoplasmic precursor and mature microRNA using gold nanoparticle and graphene oxide composite probes. Anal Chim Acta. 2018; 1021:129-139. DOI: 10.1016/j.aca.2018.03.010. View

16.

Turner A . Biosensors: sense and sensibility. Chem Soc Rev. 2013; 42(8):3184-96. DOI: 10.1039/c3cs35528d. View

17.

Lai M, Slaughter G . Label-Free MicroRNA Optical Biosensors. Nanomaterials (Basel). 2019; 9(11). PMC: 6915498. DOI: 10.3390/nano9111573. View

18.

Tricoli J, Jacobson J . MicroRNA: Potential for Cancer Detection, Diagnosis, and Prognosis. Cancer Res. 2007; 67(10):4553-5. DOI: 10.1158/0008-5472.CAN-07-0563. View

19.

Michael A, Bajracharya S, Yuen P, Zhou H, Star R, Illei G . Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis. 2009; 16(1):34-8. PMC: 2844919. DOI: 10.1111/j.1601-0825.2009.01604.x. View

20.

Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H . Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 2004; 64(11):3753-6. DOI: 10.1158/0008-5472.CAN-04-0637. View