Exosomes from Human Saliva As a Source of MicroRNA Biomarkers

Overview

Journal Oral Dis

Specialty Dentistry

Date 2009 Jul 25

PMID 19627513

Citations 359

Authors

A Michael

S D Bajracharya

P S T Yuen

H Zhou

R A Star

G G Illei

I Alevizos

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications.

Design: Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays.

Results: We successfully isolated exosomes from human saliva from healthy controls and a patient with Sjögren's syndrome. microRNAs extracted from the exosomal fraction were sufficient for quantitative PCR and microarray profiling.

Conclusions: The isolation of miRNAs from easily and non-invasively obtained salivary exosomes with subsequent characterization of the miRNA expression patterns is promising for the development of future biomarkers of the diagnosis and prognosis of various salivary gland pathologies.

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