Context-dependent Redesign of Robust Synthetic Gene Circuits

Overview

Journal Trends Biotechnol

Specialty Biochemistry

Date 2024 Feb 6

PMID 38320912

Authors

Austin Stone

Abdelrahaman Youssef

Sadikshya Rijal

Rong Zhang

Xiao-Jun Tian

Affiliations

Soon will be listed here.

Abstract

Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit-host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit-host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors. We also emphasize control strategies for mitigating these unwanted effects. This review summarizes the latest advances and the current state of host-aware and resource-aware design of synthetic gene circuits.

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