Quantifying Cellular Capacity Identifies Gene Expression Designs with Reduced Burden

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2015 Apr 8

PMID 25849635

Citations 190

Authors

Francesca Ceroni

Rhys Algar

Guy-Bart Stan

Tom Ellis

Affiliations

Soon will be listed here.

Abstract

Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.

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