PVT1 Promotes Proliferation and Macrophage Immunosuppressive Polarization Through STAT1 and CX3CL1 Regulation in Glioblastoma Multiforme

Overview

Journal CNS Neurosci Ther

Specialties Neurology
Pharmacology

Date 2024 Jan 30

PMID 38287522

Authors

Lijie Huang

Zheng Wang

Chihyi Liao

Zheng Zhao

Hua Gao

Ruoyu Huang

Jing Chen

Fan Wu

Fan Zeng

Ying Zhang

Tao Jiang

Huimin Hu

Affiliations

Soon will be listed here.

Abstract

Aims: This study aimed to investigate the role of plasmacytoma variant translocation 1 (PVT1), a long non-coding RNA, in glioblastoma multiforme (GBM) and its impact on the tumor microenvironment (TME).

Methods: We assessed aberrant PVT1 expression in glioma tissues and its impact on GBM cell growth in vitro and in vivo. Additionally, we investigated PVT1's role in influencing glioma-associated macrophages. To understand PVT1's role in cell growth and the immunosuppressive TME, we performed a series of comprehensive experiments.

Results: PVT1 was overexpressed in GBM due to copy number amplification, correlating with poor prognosis. Elevated PVT1 promoted GBM cell proliferation, while its downregulation inhibited growth in vitro and in vivo. PVT1 inhibited type I interferon-stimulated genes (ISGs), with STAT1 as the central hub. PVT1 correlated with macrophage enrichment and regulated CX3CL1 expression, promoting recruitment and M2 phenotype polarization of macrophages. PVT1 localized to the cell nucleus and bound to DHX9, enriching at the promoter regions of STAT1 and CX3CL1, modulating ISGs and CX3CL1 expression.

Conclusion: PVT1 plays a significant role in GBM, correlating with poor prognosis, promoting cell growth, and shaping an immunosuppressive TME via STAT1 and CX3CL1 regulation. Targeting PVT1 may hold therapeutic promise for GBM patients.

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