A Label-Free Aptasensor for Turn-On Fluorescent Detection of Aflatoxin B1 Based on an Aggregation-Induced-Emission-Active Probe and Single-Walled Carbon Nanohorns

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Jan 17

PMID 38231791

Authors

Huanhuan Yang

Lei Lv

Mengyu Niu

Dongjie Zhang

Zhijun Guo

Affiliations

Soon will be listed here.

Abstract

The determination of the aflatoxin B1 (AFB1) content has received widespread attention in the context of food safety, which is a global public health issue. Accordingly, a label-free and turn-on fluorescent AFB1 determination method is developed herein with an ss-DNA aptamer as the recognition element, 4, 4-(1E,1E)-2, 2-(anthracene-9, 10-diyl) bis(ethene-2, 1-diyl) bis(N, N, N-trimethylbenzenaminium iodide) (DSAI) as the aggregation-induced emission (AIE) fluorescent probe, and single-walled carbon nanohorns (SWCNHs) as the selective part with a fluorescence quenching effect. In the presence of AFB1, the AFB1-specific aptamer undergoes a structural transformation and switches from being a random helix to a folded structure. DSAI's fluorescence is protected as a result of the resistance of the transformed aptamer adsorbed on the SWCNHs' surface. Because DSAI's fluorescence is not quenchable, the fluorescence intensity is calculated as a function of the AFB1 concentration. By simply mixing DSAI, aptamer, AFB1 samples, and SWCNHs, the method can be carried out in 2 h, with a limit of detection (LOD) of 1.83 ng/mL. It has a high selectivity in the presence of other mycotoxins, and its performance is confirmed in soybean sauce with a known concentration of AFB1. The LOD was 1.92 ng/mL in the soy sauce samples and the recovery ranged from 95 to 106%, implying that the presented aptasensor has great potential for food analysis.

Citing Articles

Advances in Aptamer-Based Conjugate Recognition Techniques for the Detection of Small Molecules in Food.

Deng X, Ma B, Gong Y, Li J, Zhou Y, Xu T Foods. 2024; 13(11).

PMID: 38890976 PMC: 11172347. DOI: 10.3390/foods13111749.

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